Technical Data
Internexin, alpha
Alpha-Internexin is a Class IV intermediate filament subunit expressed very early in neuronal development. It is related to but distinct from the better known neurofilament triplet proteins, NF-L, NF-M and NF-H, having similar protein sequence motifs and a similar intron organization. Many classes of mature neurons contain a-internexin in addition to NF-L, NF-M and NF-H. In some mature neurons a-internexin is the only neurofilament subunit expressed. Antibodies to internexin are therefore unique probes to study and classify neuronal types and follow their processes in sections and in tissue culture. In addition the very early developmental expression of a-internexin means its presence is an early and convenient diagnostic feature of neuronal progenitors cells and other cell committed to the neuronal lineage.

Suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence. Other applications not tested.

Recommended Dilution:
Immunohistochemistry: 1:500 Formalin-fixed paraffin-embedded histological sections, cryostat sections.
Western Blot: 1:2500. Detects a band at 66kD.
Immunofluorescence: 1:500
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
100ul4C (-20C Glycerol)Blue IceRatRabbit
Not determined
Purified recombinant rat a-internexin
Supplied as a liquid in PBS, pH7.6, 0.05% sodium azide, 0.01% thimerosal, 15mg/ml BSA before the addition of glycerol to 40%.
Recognizes a-internexin. Species Crossreactivity: Human and other mammalian samples.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Evans, J. et al J. Neurophysiol. In press.