Technical Data
I8442-74A
Internexin, alpha
Description:
Alpha-internexin is a, 66kD, type IV intermediate filament protein(1) that is expressed in neurons during development of the peripheral and central nervous systems, as well as in a few neurons of the adult central nervous system.(2) Recent studies have demonstrated that during neuronal development, alpha-internexin replaces type VI nestin and type III vimentin intermediate filament proteins.(3) Clinical studies have also demonstrated that alpha-internexin may play a role in the development of neuronal polarity.(4)

Applications:
Suitable for use in ELISA, Western Blot, Immunoflourescence and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
ELISA: 0.1ug/ml
Western Blot: 0.1-0.5ug/ml
Immunohistochemistry (Formaldehyde fixed paraffin sections): 1-5ug/ml. Pretreat FFPE tissues with HIER using citrate buffer, pH 6.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
TypeIsotypeCloneGrade
MabIgG13G169Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceRatMouse
Concentration:
0.5mg/ml
Immunogen:
Full-length recombinant rat alpha-internexin protein
Purity:
Purified from mouse ascites fluid.
Form
Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.
Specificity:
Recognizes rat alpha-internexin at ~66kD. Species Crossreactivity: human, mouse and rabbit. Stains human neuron and nerve fiber in human cerebral cortex. Also stained glioma, ganglioneuroma, pheochromocytoma and paraganglioma in human tissue.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Pachter JS, Liem RK. J Cell Biol. 101(4):1316-22 (1985). 2. Kaplan, M.P., et. al. J Neurosci. 10(8):2735-2748 (1990). 3. Steinert P.M., et al. J Biol Chem. 274(3):1657-1666 (1999). 4. Shea T.B., Beermann M.L. Cell Motil Cytoskeleton. 43(4):322-333 (1999).