		Technical Data
I8442-74E	Internexin, alpha (66kD Neurofilament Protein, Alpha Inx, INA, Internexin Neuronal intermediate Filament Protein alpha, MGC12702, NEF 5, NEF5, Neurofilament 5 (66kD), Neurofilament 66, Neurofilament 66 Tax Binding Protein, NF 66, NF66, TXBP 1, TXBP1)

Description:
Alpha-internexin is a Class IV intermediate filament originally discovered as it co-purifies with other neurofilament subunits (1). Alpha-internexin is related to but distinct from the better known neurofilament triplet proteins, NF-L, NF-M and NF-H, having similar protein sequence motifs and a similar intron organization. It is expressed only in neurons and in large amounts early in neuronal development, but is down-regulated in many neurons as development proceeds. Many classes of mature neurons contain alpha-internexin in addition to NF-L, NF-M and NF-H. In some mature neurons alpha-internexin is the only neurofilament subunit expressed. Antibodies to alpha-internexin are therefore unique probes to study and classify neuronal types and follow their processes in sections and in tissue culture. In addition, recent studies show a marked up-regulation of alpha-internexin during neuronal regeneration (2). The use of antibodies to this protein in the study of brain tumors has not been examined to date, but is likely to be of interest. Recently Cairns et al. used this antibody to show that alpha-internexin is an abundant component of the inclusions of neurofilament inclusion body disease (NFID), a serious human neurodegenerative disorder (3,4). The antibody was also used to confirm the presence of circulating auto-antibodies to alpha-internexin in the sera of some patients with endocrine autoimmunity, as well as in some normal individuals (5).

Applications:
Suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence. Other applications not tested.

Recommended Dilution:
Immunofluorescence: 1:250
Western Blot: 1:2000
Immunohistochemistry (PEFF or frozen): 1:1000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 3 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Pab	IgG		Supernatant

Size		Storage	Shipping	Source	Host
100ul		-20°C	Blue Ice	Rat	Mouse

Concentration:

Not determined.

Immunogen:

Recombinant rat alpha-internexin expressed in and purified from E. coli.

Purity:

Tissue culture supernatant

Form

Supplied as a liquid in 10mM sodium azide.

Specificity:

Recognizes the ~66kD rat alpha Internexin protein. Epitope: C-terminal non-helical extension of alpha Internexin.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Pachter, J and Liem, RKH. Alpha-Internexin, a 66-kD intermediate filament-binding protein from mammalian central nervous tissues. J Cell Biol 101:1316-22 (1985). 2. McGraw et al. Axonally transported peripheral signals regulate alpha-internexin expression in regenerating motoneurons. J Neurosci 22:4955-63 (2002). 3. Cairns NJ et al. alpha-Internexin aggregates are abundant in neuronal intermediate filament inclusion disease (NIFID) but rare in other neurodegenerative diseases. Acta Neuropathol (Berl). May 28 [Epub ahead of print] (2004). 4. Cairns NJ et al. alpha-internexin is present in the pathological inclusions of neuronal intermediate filament inclusion disease. Am J Pathol. 164:2153-61 (2004). 5. Rajasalu T, Teesalu K, Janmey PA, Uibo R. Demonstration of natural autoantibodies against the neurofilament protein alpha-internexin in sera of patients with endocrine autoimmunity and healthy individuals. Immunol Lett. 94:153-60 (2004).