Technical Data
I8442-74H
Internexin, alpha (66kD Neurofilament Protein, Alpha Inx, INA, Internexin Neuronal intermediate Filament Protein alpha, MGC12702, NEF 5, NEF5, Neurofilament 5 (66kD), Neurofilament 66, Neurofilament 66 Tax Binding Protein, NF 66, NF66, TXBP 1, TXBP1)
Description:
Neurofilament-66, also known as a-internexin, is a member of the IF family. It is found in developing neuroblasts and in the CNS of adults. Neurofilament-66 is expressed in the adult brain at lower levels than other neurofilament proteins.

Applications:
Suitable for use in Flow Cytometry, Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 1:25
Western Blot: 1:5000-10000
Immunohistochemistry (paraffin): 1:250-500
Immunocytochemistry: 1:250-500
Immunoprecipitation: 1:50
Optimal dilutions to be determined by the researcher.

Positive Control:
SHSY5Y cell lysate

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG9i172Supernatant
SizeStorageShippingSourceHost
100ul-20°CBlue IceHumanRabbit
Concentration:
Not determined
Immunogen:
Synthetic peptide corresponding to residues in the tail domain of human Neurofilament-66. Epitope: Tail Domain
Purity:
Supernatant
Form
Supplied as a liquid in 50mM Tris-Glycine, pH 7.4, 0.15M sodium chloride, 0.01% sodium azide, 0.05% BSA, 40% glycerol.
Specificity:
Recognizes human Neurofilament-66 at and around the tail domain. Species Crossreactivity: rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.