		Technical Data
I8442-74M	Internexin, alpha, ID (I305) (INA, 66kD Neurofilament Protein, Alpha-Internexin, Alpha Inx, FLJ18662, FLJ57501, MGC12702, NEF 5, NEF5, Neurofilament 5, Neurofilament 66, Neurofilament-66 NF 66, NF66, NF-66)

Description:
INA is a class-IV neuronal intermediate filament that is able to self-assemble. It is involved in the morphogenesis of neurons. It may form an independent structural network without the involvement of other neurofilaments or it may cooperate with NF-L to form the filamentous backbone to which NF-M and NF-H attach to form the cross-bridges.

Applications:
Suitable for use in ELISA, Western Blot, and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
ELISA: 1:1,000
Western Blot: 1:50-1:100
Immunohistochemistry: 1:50-1:100
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab	IgG		Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Rabbit

Concentration:

~0.25mg/ml

Immunogen:

Synthetic peptide selected from the Center region (I305) of human INA (KLH).

Purity:

Purified by ammonium sulfate precipitation.

Form

Supplied as a liquid in PBS, 0.09% sodium azide.

Specificity:

Recognizes human INA.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1.Armstrong,R.A.,Eur. J. Neurol. 13(5), 528-532 (2006). 2.Suzuki,T., Eur. J. Neurosci. 21(2), 339-350 (2005) 3.Cairns,N.J., Am. J. Pathol. 164(6), 2153-2161 (2004).