		Technical Data
I8442-74Q	Internexin, alpha (66kD Neurofilament Protein, Alpha Inx, INA, Internexin Neuronal intermediate Filament Protein alpha, MGC12702, NEF 5, NEF5, Neurofilament 5 (66kD), Neurofilament 66, Neurofilament 66 Tax Binding Protein, NF 66, NF66, TXBP 1, TXBP1)

Description:
Neurofilaments are the intermediate filaments found in neuronal cells. They are composed of a combination of subunit proteins which typically includes the neurofilment triplet proteins, NF-L, NF-M, and NF-H. Neurofilaments may also include peripherin, alpha-internexin (NF66), nestin, and vimentin. Neurofilament proteins are useful biomarkers as their expression can vary during neuronal development and differentiation, between between neuronal cell subtypes, and be altered during pathological states. Alpha-internexin/NF66, related to the neurofilament triplet proteins, is expressed during early neuronal development. The temporal and spatial expression pattern of alpha-internexin varies between different subtypes of differentiating and mature neurons. An upregulation of alpha-internexin has been observed during neuronal regeneration. Abnormal neuronal aggregates of alpha-internexin along with N-L, NF-M, and NF-H have been identified as the pathological hallmarks of the neuronal intermediate filament inclusion disease (NIFID), an early onset neurodegenerative disease associated with dementia. Antibodies to alpha-internexin are useful probes for studying and classifying neuronal subtypes, and for identifying neuronal progenitor cells and other cells committed to the neuronal lineage.

Applications:
Suitable for use in Immunofluorescence, Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Immunofluorescence: 1:100-1:1000
Western Blot: 1:500-1:2500
Immunohistochemistry (frozen, formalin-fixed, paraffin-embeded tissue sections, formalin-fixed tissue cultured cells): 1:2000-1:5000
Immunocytochemistry: 1:100-1:1000
Optimal dilutions to be determined by the researcher.

Positive Control:
PC12 cells, brain, spinal cord, or nerve tissue

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Mab	IgG1	11E243	Supernatant

Size		Storage	Shipping	Source	Host
100ul		-20°C	Blue Ice	Rat	Mouse

Concentration:

Not determined.

Immunogen:

Recombinant corresponding to rat alpha internexin.

Purity:

Supernatant

Form

Supplied as a liquid, 0.01% thimerosal, 1mg/ml BSA, 50% glycerol.

Specificity:

Recognizes rat Internexin, alpha. Species Crossreactivity: human. Species sequence homology: most mammals.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Julien, J.P., R.C. Lariviere. 2004. J. Functions of intermediate filaments in neuronal development and disease. J Neurobiol. 58:131-148. 2. Cairns, N.J., V. Zhukareva, K. Uryu, B. Zhang et al. 2004. Alpha-internexin is present in the pathological inclusions of neuronal intermediate filament inclusion disease. Am J Pathol. 164:2153-2161.