Technical Data
I8442-78A
Internexin, alpha (NF66)
Description:
alpha-internexin is a ~66 kD Class IV intermediate filament originally discovered as it copurifies with other neurofilament subunits. It is related to but distinct from the better known neurofilament triplet proteins, NF-L, NF-M and NF-H, having similar protein sequence motifs and a similar intron organization. It is expressed in large amounts early in neuronal development, but is downregulated in many neurons as development procedes. Many classes of mature neurons contain alpha-internexin in addition to NF-L, NF-M anbd NF-H. In some mature neurons alpha-internexin is the only neurofilament subunit expressed. Antibodies to internexin are therefore unique probes to study and classify neuronal types and follow their processes in sections and in tissue culture. In addition the very early developmental expression of alpha-internexin means its presence is an early and convenient diagnostic feature of neuronal progenitors cells and other cell committed to the neuronal lineage.

Applications:
Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:10,000
Immunohistochemistry: 1:1000
Immunocytochemistry: 1:1000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgYPurified
SizeStorageShippingSourceHost
100ul-20CBlue IceChicken
Concentration:
Not determined
Immunogen:
Polyclonal recombinant alpha-internexin
Purity:
Purified immunoglobulin
Form
Supplied as a liquid.
Specificity:
Recognizes alpha-Internexin. Species Crossreactivity: Human, mouse and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.