Technical Data
Internexin, alpha (NF66)
Neurofilaments (NF) are cytoplasmic intermediate filaments (IFs) found in neurons. NF-66, also known as -internexin, is a member of the IF family. Classified as a type IV IF, NF-66 is expressed by most neurons and has been shown to co-assemble with other NF triple proteins (light, medium and heavy NF) (1-2). In the adult brain, NF-66 is expressed at relatively low levels compared to the other NF proteins (3). NF-66 as also been shown to assemble into 10mm diameter IF in vitro.

Suitable for use in Flow Cytometry, Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 1:25
Western Blot: 1:5,000-10,000
Immunohistochemistry: 1:250-500
Immunocytochemistry: 1:250-500
Immunoprecipitation: 1:50

Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Freeze at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Manufactured incorporating RabMAb technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
100ul -20CBlue IceHumanRabbit
A synthetic peptide corresponding to residues in the tail domain of human NF-66
Supplied as a liquid.
Recognizes human NF-66
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Kaplan MP, Chin SSM, Fliegner KH, Liem RKH: J Neurosci 1990, 10:2735-2748 2. Ching GY, Liem RKH: J Biol Chem 1991, 29:19459-194683. Ching GY, Liem RKH: J Cell Sci 1998, 111:321-333