Technical Data
I8442-78B
Internexin, alpha (NF66)
Description:
Neurofilaments (NF) are cytoplasmic intermediate filaments (IFs) found in neurons. NF-66, also known as -internexin, is a member of the IF family. Classified as a type IV IF, NF-66 is expressed by most neurons and has been shown to co-assemble with other NF triple proteins (light, medium and heavy NF) (1-2). In the adult brain, NF-66 is expressed at relatively low levels compared to the other NF proteins (3). NF-66 as also been shown to assemble into 10mm diameter IF in vitro.

Applications:
Suitable for use in Flow Cytometry, Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 1:25
Western Blot: 1:5,000-10,000
Immunohistochemistry: 1:250-500
Immunocytochemistry: 1:250-500
Immunoprecipitation: 1:50

Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Manufactured incorporating RabMAb® technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
TypeIsotypeCloneGrade
MabIgG6k230Supernatant
SizeStorageShippingSourceHost
100ul -20°CBlue IceHumanRabbit
Concentration:
Immunogen:
A synthetic peptide corresponding to residues in the tail domain of human NF-66
Purity:
Supernatant
Form
Supplied as a liquid.
Specificity:
Recognizes human NF-66
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Kaplan MP, Chin SSM, Fliegner KH, Liem RKH: J Neurosci 1990, 10:2735-2748 2. Ching GY, Liem RKH: J Biol Chem 1991, 29:19459-194683. Ching GY, Liem RKH: J Cell Sci 1998, 111:321-333