Suitable for use in Immunofluorescence and Western Blot. Other applications not tested.
Western Blot: 1:5000-1:10,000
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
|Recombinant alpha-internexin expressed in and purified from E. coli|
|Purified by immunoaffinity chromatography.|
|Supplied as a liquid in PBS, pH 7.4, 0.05% sodium azide.|
|Recognizes alpha-internexin/NF66 in human, rat, mouse, cat and other mammals.|
|Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.|
1. Cairns NJ, et al. alpha-internexin is present in the pathological inclusions of neuronal intermediate filament inclusion disease. Am. J. Pathol. Jun 2004; 164(6): 2153-2161. 2. Behrends U, et al. Novel products of the HUD, HUC, NNP-1 and alpha-internexin genes identified by autologous antibody screening of a pediatric neuroblastoma library. Int. J. Cancer. Aug 2002; 100(6): 669-677.|