Technical Data
I8442-78J
Internexin, alpha (INa, NF66, 66kD Neurofilament Protein, Alpha Inx, Internexin Neuronal Intermediate Filament Protein alpha, MGC12702, Neurofilament 5 (66kD), NEF5, Neurofilament 66, Neurofilament 66 Tax Binding Protein, TXBP1)
Description:
Neurofilaments (NF) are cytoplasmic intermediate filaments (IFs) found in neurons. NF-66, also known as alpha-internexin, is a member of the IF family. Classified as a type IV IF, NF-66 is expressed by most neurons and has been shown to co-assemble with other NF triple proteins (light, medium and heavy NF) (1-2). In the adult brain, NF-66 is expressed at relatively low levels compared to the other NF proteins (3). NF-66 as also been shown to assemble into 10mm diameter IF in vitro

Applications:
Suitable for use in Flow Cytometry, Western Blot and Immunoprecipitation. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 1:20
Western Blot: 1:50,000
Immunoprecipitation: 1:100
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanRabbit
Concentration:
Not determined
Immunogen:
Synthetic peptide corresponding to residues on the C-terminus of human NF-66.
Purity:
Purified by Immunoaffinity chromatography.
Form
Supplied as a liquid in 50 mM Tris-Glycine (pH 7.4), 0.15 M sodium chloride, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Specificity:
Recognizes human Neurofilament 66. Species Crossreactivity: Mouse and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Kaplan MP, Chin SSM, Fliegner KH, Liem RKH: J Neurosci 1990, 10:2735-2748 2. Ching GY, Liem RKH: J Biol Chem 1991, 29:19459-68 3. Ching GY, Liem RKH: J Cell Sci 1998, 111:321-333