Technical Data
I8443-75L
IL-27 (Interleukin-27 Subunit alpha, IL-27 Subunit alpha, IL-27-A, IL27-A, p28, IL27A)
Description:
Recently, a new heterodimeric cytokine termed IL-27 that consists of EBI3 (Epstein-Barr virus-induced gene 3), an IL-12p40-related protein, and p28, a newly discovered IL-12p35-related polypeptide has been discovered0. IL-27 is involved in early Th1 initiation. Its action is mediated through the orphan cytokine receptor WSX-1/TCCR and gp130. IL-27 may also play a role in the induction of T-bet expression and regulation of Ig class switching in B cells.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1-2ug/ml
Optimal dilutions to be determined by the researcher.

Positive Control:
Jurkat, NIH 3T3

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanRabbit
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide corresponding to aa31-46 (RPPGRPQLSLQELRRE) of human IL-27 (p28 subunit) of (Genbank accession number NP_663634.)
Purity:
Purified by Protein G affinity Chromatography.
Form
Supplied as a liquid in PBS, 0.2% gelatin, 0.05% sodium azide.
Specificity:
Recognizes human IL-27. Species Crossreactivity: mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Pflanz S, Timans JC, Cheung J, et al. Immunity 16(6): 779-790 (2002). 2. Brombacher F, Kastelein RA, Alber G. Trends Immunol. 24(4):207-212 (2003). 3. Yoshimoto T, Okada K, Morishima N, Kamiya S, et al. J Immunol. 173(4):2479-2485 (2004).