Technical Data
I8443-75M
IL-27 (Interleukin-27 Subunit alpha, IL-27 Subunit alpha, IL-27-A, IL27-A, p28, IL27A)
Description:
IL-27 is a 243aa novel heterodimeric cytokine belonging to the IL-6/IL-12 family and produced by activated antigen-presenting cells. It is composed of two subunits, Epstein-Barr virus (EBV)-induced gene 3 (EBI3) and p28. Recent reports suggest that IL-27 is a potent inhibitor of HIV-1 replication in PBMCs, CD4 (+) T cells and macrophages. It has antiangiogenic activity, which plays an important role in its antitumor and antimetastatic activities. It is also responsible for early regulation of Th1 adaptive immune response. It strongly synergizes with IL-12 to trigger IFN-gamma production of naive CD4 (+) T cells and drives rapid clonal expansion of naive but not memory CD4 (+) T cells through the cytokine receptor WSX-1/TCCR.

Applications:
Suitable for use in ELISA. Other applications not tested.

Recommended Dilution:
ELISA: 1:100-1:1000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGSerum
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanRabbit
Concentration:
Not determined.
Immunogen:
Synthetic peptide corresponding to aa95-107 (QAWRRLSDPERLC) of human IL-27.
Purity:
Serum
Form
Supplied as a liquid, 0.025% sodium azide, 50% glycerol.
Specificity:
Recognizes human IL-27. Species Crossreactivity: chimpanzee. Species sequence homology: bovine, canine, mouse and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Pflanz, S. et al. Immunity. 16:779-790 (2002). 2. Lucas, S. et al. Proc. Natl. Acad. Sci. USA. 100:15047-15052 (2003). 3. Larousserie, F. et al. Am. J. Pathol. 166:1217-1228 (2005). 4. Fakruddin, JM. et al. Blood. 109:1841-1849 (2007).