Technical Data
IP-10, Recombinant, Human (Interferon gamma Inducible Protein, CXCL10)
Molecular Biology Storage: -20CShipping: Blue Ice
Among CXC chemokines, CXCL10 has been identified to play an important role in several endocrinological autoimmune diseases such as Hashimoto's thyroiditis, Graves'disease and Type 1 diabetes mellitus.The chemokine IP-10 (interferon-inducible protein of 10 kD, CXCL10) is a chemoattractant for CXCR3+ T cells, binds to the G protein-coupled receptor CXCR3, which is found mainly on activated T cells and NK cells, and plays an important role in Th1-type inflammatory diseases. IP-10 also binds to glycosaminoglycans (GAGs), an interaction thought to be important for its sequestration on endothelial and other cells

Recombinant Human IP-10 produced in E.Coli is a single,non-glycosylated, polypeptide chain containing 77 amino acids and having a molecular mass of 8516 Dalton. Recombinant Human IP-10 is purified by proprietary chromatographic techniques.

Amino Acid Sequence:
The first five N-terminal amino acids is Val-Pro-Leu-Ser-Arg.

Biological Activity:
Recombinant Human IP-10 is fully biologically active when compared to standard. The Biological activity is calculated by its ability to chemoattract human T lymphocytes at 10-100.0 ng/ml.

0.1ng/ug (IEU/ug) of IP-10.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 3 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
Source: E. coli
Purity: 98% as determined by RP-HPLC, and/or reducing and non-reducing SDS-PAGE Silver Stained gelEndotoxin:
0.1ng/ug (IEU/ug).
Concentration: As reported
Form: Supplied as a lyophilized powder without additives. Reconstitute with sterile, ddH2O, 0.1% BSA or HSA to 100ug/ml

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Early post-transplant urinary IP-10 expression after kidney transplantation is predictive of short- and long-term graft function. Kidney Int 2006 May;69(9):1683-90 2. IP-10 blocks vascular endothelial growth factor-induced endothelial cell motility and tube formation via inhibition of calpain. Circ Res 2006 Mar 17;98(5):617-25 3. Compartment-specific expression and function of the chemokine IP-10/CXCL10 in a model of renal endothelial microvascular injury. J Am Soc Nephrol 2006 Feb;17(2):454-64 4. IP-10/MCP-1 ratio in CSF is an useful diagnostic marker of neuropsychiatric lupus patients. Rheumatology (Oxford) 2006 Feb;45(2):232-4 5. T lymphocyte chemotactic chemokines in acute myelogenous leukemia (AML): local release by native human AML blasts and systemic levels of CXCL10 (IP-10), CCL5 (RANTES) and CCL17 (TARC). Cancer Immunol Immunother 2006 Jul;55(7):830-40 6. TLR3-mediated signal induces proinflammatory cytokine and chemokine gene expression in astrocytes: differential signaling mechanisms of TLR3-induced IP-10 and IL-8 gene expression. Glia 2006 Feb;53(3):248-56

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.