Technical Data
I8449-10C
IP-10, Mouse, Recombinant
5ug
25ug
100ug
Growth Factors, Cytokines Storage: -70CShipping: Blue Ice
CXCL10, a CXC chemokine, has been identified to play an important role in several endocrinological autoimmune diseases such as Hashimoto's thyroiditis, Graves' disease and Type 1 diabetes mellitus. The chemokine IP-10 (interferon-inducible protein of 10kD, CXCL10) is a chemoattractant for CXCR3+ T cells, binds to the G protein-coupled receptor CXCR3, which is found mainly on activated T cells and NK cells, and plays an important role in Th1-type inflammatory diseases. IP-10 also binds to glycosaminoglycans (GAGs), an interaction thought to be important for its sequestration on endothelial and other cells.

Recombinant mouse IP-10 is a single, non-glycosylated, polypeptide chain containing 77 amino acids and having a molecular mass of 8.7kD.

Biological Activity:
Murine IP-10 is fully biologically active when compared to standard. The Biological activity is calculated by its ability to chemoattract IL-2 activated T cells at 0.1-10ng/ml.

AA Sequence:
IPLARTVRCN CIHIDDGPVR MRAIGKLEII PASLSCPRVE IIATMKKNDE QRCLNPESKT IKNLMKAFSQ KRSKRAP

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O, 0.1% HSA or BSA. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
8.7kD
Source: Recombinant, murine, E. coli
Purity: 97% as determined by RP-HPLC and reducing and non-reducing SDS-PAGE. Endotoxin: 0.1EU/ug (0.01ng/ug, LAL)
Form: Supplied as a lyophilized powder. Reconstitute with sterile dH2O, 0.1% BSA or HSA to 0.1mg/ml, which can be further diluted into other aqueous solutions.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Pediatr. Res. 61(1): 93-98 (2007). 2. J. Vasc. Res. 44(1): 51-60 (2007). 3. Hepatology 44(6): 1617-1625 (2006). 4. J. Interferon Cytokine Res. 26(10): 699-705 (2006). 5. J. Leukoc. Biol. 80(6): 1364-1374 (2006). 6. Circulation 114(12): 1293-1300 (2006).

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.