Technical Data
I8449-12
IP-10, Recombinant, Mouse (Gamma Interferon Inducible Protein-10)
10ug
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Technical Data |
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I8449-12 |
IP-10, Recombinant, Mouse (Gamma Interferon Inducible Protein-10) |
10ug |
| Growth Factors, Cytokines | Storage: -20°CShipping: Blue Ice |
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Source: Recombinant corresponding to mouse IP-10 expressed in E. coli. Molecular Weight: ~8.7kD Activity: ED50 range = 25.0-100ng/ml, determined by the dose dependent chemotaxis of human lymphocytes cultured in the presence of IL-2. The optimal concentration should be determined for each specific application by a dose response assay. Endotoxin: ~0.1ng/ug Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Molecular Weight: ~8.7kD |
Source: E. coli Purity: ~95% (SDS-PAGE) Concentration: Not determined Form: Supplied as a lyophilized powder. BSA free. Reconstitute with sterile ddH2O to 0.1-1mg/ml. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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1. Ohmori, Y. and T.A. Hamilton (1990) A macrophage LPS-inducible early gene encodes the murine homologue of IP-10. Biochem. Biophys. Res. Commun. 168:1261-1267. 2. Vanguri, P. and J.M. Farber (1990) Identification of CRG-2. An interferon-inducible mRNA predicted to encode a murine monokine. J. Biol. Chem. 265:15049-15057. 3. Loetscher, M., B. Gerber, P. Loetscher, S.A. Jones, L. Piali, I. Clark-Lewis, M. Baggiolini, and B. Moser (1996) Chemokine receptor specific for IP10 and mig: structure, function, and expression in activated T-lymphocytes. J. Exp. Med. 184:963-969. 4. Sgadari, C., A. Angiolillo, B.W. Cherney, S.E. Pike, J.M. Farber, L.G. Koniaris, P. Vanguri, P.R. Burd, N. Sheikh, G. Gupta, J. Teruya-Feldstein, and G. Tosato (1996) 5. Interferon-inducible protein-10 identified as a mediator of tumor necrosis in vivo. Proc. Nat’l. Acad. Sci. USA 93:13791-13796. 6. Farber, J.M. (1997) Mig and IP-10: CXC chemokines that target lymphocytes. J. Leukoc. Biol. 61:246-257.
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