Technical Data
I8449-12
IP-10, Recombinant, Mouse (Gamma Interferon Inducible Protein-10)
10ug
Growth Factors, Cytokines Storage: -20CShipping: Blue Ice
Source:
Recombinant corresponding to mouse IP-10 expressed in E. coli.

Molecular Weight:
~8.7kD

Activity:
ED50 range = 25.0-100ng/ml, determined by the dose dependent chemotaxis of human lymphocytes cultured in the presence of IL-2. The optimal concentration should be determined for each specific application by a dose response assay.

Endotoxin: ~0.1ng/ug

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
~8.7kD
Source: E. coli

Purity: ~95% (SDS-PAGE)
Concentration: Not determined
Form: Supplied as a lyophilized powder. BSA free. Reconstitute with sterile ddH2O to 0.1-1mg/ml.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Ohmori, Y. and T.A. Hamilton (1990) A macrophage LPS-inducible early gene encodes the murine homologue of IP-10. Biochem. Biophys. Res. Commun. 168:1261-1267. 2. Vanguri, P. and J.M. Farber (1990) Identification of CRG-2. An interferon-inducible mRNA predicted to encode a murine monokine. J. Biol. Chem. 265:15049-15057. 3. Loetscher, M., B. Gerber, P. Loetscher, S.A. Jones, L. Piali, I. Clark-Lewis, M. Baggiolini, and B. Moser (1996) Chemokine receptor specific for IP10 and mig: structure, function, and expression in activated T-lymphocytes. J. Exp. Med. 184:963-969. 4. Sgadari, C., A. Angiolillo, B.W. Cherney, S.E. Pike, J.M. Farber, L.G. Koniaris, P. Vanguri, P.R. Burd, N. Sheikh, G. Gupta, J. Teruya-Feldstein, and G. Tosato (1996) 5. Interferon-inducible protein-10 identified as a mediator of tumor necrosis in vivo. Proc. Natl. Acad. Sci. USA 93:13791-13796. 6. Farber, J.M. (1997) Mig and IP-10: CXC chemokines that target lymphocytes. J. Leukoc. Biol. 61:246-257.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.