Technical Data
I8500
IPTG Dioxane Free (Isopropyl-ß-D-thiogalactoside(galactopyranoside))
1g
5g
10g
100g
250g
500g
5g
10g
100g
250g
500g
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Technical Data |
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I8500 |
IPTG Dioxane Free (Isopropyl-ß-D-thiogalactoside(galactopyranoside)) |
1g 5g 10g 100g 250g 500g |
| Biochemicals | Storage: -20°CShipping: RT |
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IPTG is a carbohydrate used to induce b-galactosidase for the selection of recombinant plasmids. Used to select for lac Y mutants and to induce the lac operon in E. coli. IPTG will also induce the cellular content of lactose permease. Solubility: Colorless, clear, complete Identity (IR): Conforms to structure pH (5%): 5.0-7.0 Dioxane: Not Detected Specific Rotation (C=1,H2O): -29° to -35° Melting Point: 109 -114.0°C Water (KF): 1.0% Blue-White Assay: Induces b-galactosidase in a pUC 18-containing strain. Storage and Stability: Powder is very stable at RT. Stable for 12 months. For long-term storage of reconstituted product, aliquot and store at -20°C. Aliquots are stable for 6 months at -20°C. Further dilutions can be made in assay buffer. CAS Number: 367-93-1 Molecular Formula: C9H18O5S Molecular Weight: 238.31 |
Purity (HPLC): 99% Form: White crystalline powder. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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US Biological application reference: 1. Alag, R. et al., (2010) Protein Science 19; 1577–1586. 2. Norgaard, P. & Winther, J.R., Biochem J. 358: 269–274 (2001). 3. Dennis, A.M., et al., Bioconjugate Chem. June 22, epub (2010). 1. Maniatis, et al., Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N. Y., 2nd Ed., 4: 22-8.57 (1987).
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