IPTG Dioxane Free (Isopropyl-ß-D-thiogalactoside(galactopyranoside))
|Biochemicals||Storage: -20°CShipping: RT|
IPTG is a carbohydrate used to induce b-galactosidase for the selection of recombinant plasmids. Used to select for lac Y mutants and to induce the lac operon in E. coli. IPTG will also induce the cellular content of lactose permease.
Colorless, clear, complete
Conforms to structure
Specific Rotation (C=1,H2O):
-29° to -35°
Induces b-galactosidase in a pUC 18-containing strain.
Storage and Stability:
Powder is very stable at RT. Stable for 12 months. For long-term storage of reconstituted product, aliquot and store at -20°C. Aliquots are stable for 6 months at -20°C. Further dilutions can be made in assay buffer.
Purity (HPLC): 99%
Form: White crystalline powder.
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
US Biological application reference: 1. Alag, R. et al., (2010) Protein Science 19; 1577–1586. 2. Norgaard, P. & Winther, J.R., Biochem J. 358: 269–274 (2001). 3. Dennis, A.M., et al., Bioconjugate Chem. June 22, epub (2010). 1. Maniatis, et al., Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N. Y., 2nd Ed., 4: 22-8.57 (1987).