		Technical Data
I8601-02C	IDE, ID (Insulin-degrading Enzyme, Insulysin, Insulinase, Insulin Protease, Abeta-Degrading Protease)

Description:
IDE belongs to a protease family responsible for intercellular peptide signalling. Though its role in the cellular processing of insulin has not yet been defined, insulin-degrading enzyme is thought to be involved in the termination of the insulin response.

Applications:
Suitable for use in ELISA, Western Blot, and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
ELISA: 1:1,000
Western Blot: 1:50-1:100
Immunohistochemistry: 1:50
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab	IgG		Affinity Purified

Size		Storage	Shipping	Source	Host
200ul		-20°C	Blue Ice	Human	Rabbit

Concentration:

~0.25mg/ml

Immunogen:

Synthetic peptide selected from the Center region of human IDE (KLH).

Purity:

Purified by Protein A affinity chromatography.

Form

Supplied as a liquid in PBS, 0.09% sodium azide.

Specificity:

Recognizes human IDE.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Vepsalainen,S.,J. Med. Genet. 44(9), 606-608 (2007). 2. Kim,M.,J. Biol. Chem. 282(11), 7825-7832 (2007). 3. Radulescu,R.T.,Int. J. Oncol. 30(1), 73-80 (2007). 4. Li,Q.,Cell 127(2), 305-316 (2006). 5. Razvan T. Radulescu, et al. Mol. Cancer Ther. published 13 October 2009, 10.1158/1535-7163.MCT-09-0706. (Applications: IP) (E-pub).