Technical Data
I8601-02H
IDE (Insulin-degrading Enzyme, Abeta-degrading Protease, Insulin Protease, Insulinase, Insulysin)
Description:
Insulin Degrading Enzyme (IDE) encodes a zinc metallopeptidase that degrades intracellular insulin, and thereby terminates insulins activity, as well as participating in intercellular peptide signalling by degrading diverse peptides such as glucagon, amylin, bradykinin, and kallidin. The preferential affinity of IDE for insulin results in insulin-mediated inhibition of the degradation of other peptides such as beta-amyloid. Deficiencies in IDE's function are associated with Alzheimer's disease and type 2 diabetes mellitus but mutations in this gene have not been shown to be causitive for these diseases. This protein localizes primarily to the cytoplasm but in some cell types localizes to the extracellular space, cell membrane, peroxisome, and mitochondrion.

Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:5000-1:20,000
Immunohistochemistry (Formalin fixed paraffin embedded): 10ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabAffinity Purified
SizeStorageShippingSourceHost
50ul-20CBlue IceHumanRabbit
Concentration:
~1mg/ml
Immunogen:
Recombinant corresponding to aa349-605 from human insulin-degrading enzyme.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in 0.1M Tris-glycine, pH 7, 0.01% thimerosal, 10% glycerol.
Specificity:
Recognizes human IDE. Species sequence homology: mouse (94%), rat (95%), bovine (98%) and zebrafish (90%).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.