Technical Data
I8700-01C
IRS-1 (Insulin Receptor Substrate 1)
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Technical Data |
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I8700-01C |
IRS-1 (Insulin Receptor Substrate 1) |
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Description: Insulin Receptor Substrate (IRS-1) is one of the major substrates of Insulin Receptor Kinase. IRS-1 contains multiple tyrosine phosphorylation motifs that serve as docking sites for SH2 domain-containing proteins (Grb2, p85, SHP-2, Fyn and others) and which mediate the metabolic and growth promoting functions of insulin. IRS-1 also contains over 30 potential Ser/Thr phosphorylation sites for kinases, such as PKA, PKC and MAPK. The phosphorylation of Ser 612 and Ser636/639 are mediated by PKA and mTOR pathways, respectively, resulting in an inhibition of insulin signaling in the cell and suggesting a potential mechanism for insulin resistance in some models of obesity. Once phosphorylated by insulin receptor, IRS-1 recruits the downstream components that mediate the insulin signaling. IRS-1 protein contains a conserved pleckstrin homology (PH) domain located at its amino terminus, adjacent to a phospho-tyrosine binding (PTB) domain. Applications: Suitable for use in Immunoprecipitation, Immunocytochemistry and Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunoprecipitation: 2-4ug immunoprecipitated IRS1 from 500ug of 3T3/A31 RIPA lysate. Immunocytochemistry: 8ug/ml stained IRS1 in mouse brain cells fixed with 10% formalin as reported by an independent laboratory. Immunohistochemistry: Reported by an independent laboratory to detect IRS1 in mouse brain sections. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
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