Technical Data
IRS-1 (Insulin Receptor Substrate 1)
Insulin Receptor Substrate (IRS-1) is one of the major substrates of Insulin Receptor Kinase. IRS-1 contains multiple tyrosine phosphorylation motifs that serve as docking sites for SH2 domain-containing proteins (Grb2, p85, SHP-2, Fyn and others) and which mediate the metabolic and growth promoting functions of insulin. IRS-1 also contains over 30 potential Ser/Thr phosphorylation sites for kinases, such as PKA, PKC and MAPK. The phosphorylation of Ser 612 and Ser636/639 are mediated by PKA and mTOR pathways, respectively, resulting in an inhibition of insulin signaling in the cell and suggesting a potential mechanism for insulin resistance in some models of obesity. Once phosphorylated by insulin receptor, IRS-1 recruits the downstream components that mediate the insulin signaling. IRS-1 protein contains a conserved pleckstrin homology (PH) domain located at its amino terminus, adjacent to a phospho-tyrosine binding (PTB) domain.

Suitable for use in Immunoprecipitation, Immunocytochemistry and
Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunoprecipitation: 2-4ug immunoprecipitated IRS1 from 500ug of 3T3/A31 RIPA lysate.
Immunocytochemistry: 8ug/ml stained IRS1 in mouse brain cells fixed with 10% formalin as reported by an independent laboratory.
Immunohistochemistry: Reported by an independent laboratory to detect IRS1 in mouse brain sections.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
MabIgG1,k5H19Affinity Purified
200ug-20CBlue IceRatMouse
Full length recombinant rat IRS-1 protein produced in baculovirus without tags (Swissprot ID# P35570).
Purified by Protein G affinity chromatography.
Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, containing 0.05% sodium azide before the addition of glycerol to 30%.
Recognizes rat IRS1, Mr 165kD. Species Crossreactivity: Mouse
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. White, M.F., Recent Prog. Horm. Res. 53: 119-138, 1998.