Technical Data
I8700-01D
IRS-1 (Insulin Receptor Substrate 1)
Description:
Insulin Receptor Substrate 1 (IRS-1) is a cytoplasmic protein that participates in the insulin signaling pathway. It is phosphorylated by the Insulin receptor upon binding of Insulin and subsequently associates with several SH2-domain-containing proteins.

Applications:
Suitable for use in Immunocytochemistry, Western Blot and ELISA. Other applications not tested.

Recommended Dilution:
Immunocytochemistry: 10ug/ml
Western Blot: 1-2ug/ml
ELISA: 0.5-1ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG17H204Affinity Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceHumanMouse
Concentration:
As reported
Immunogen:
E. coli-derived recombinant human IRS-1 (aa801-1020) Accession # P35568
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a lyophilized powder from a 0.2um filtered solution in PBS, 5% trehalose. Reconstitute with 1ml sterile ddH2O or sterile 40-50% glycerol.
Specificity:
Recognizes human IRS-1.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.