Technical Data
IRS-1 (Insulin Receptor Substrate 1)
Insulin receptor substrate 1 (IRS-1) is one of the major substrates of the insulin receptor kinase. IRS-1 contains multiple tyrosine phosphorylation motifs that serve as docking sites for SH2 domain containing proteins, which mediate the metabolic and growth promoting functions of insulin. IRS-1 also contains over 30 potential serine/threonine phosphorylation sites. Ser307 of IRS-1 is phosphorylated by JNK and IKK and Ser789 is phosphorylated by SIK-2, a member of AMPK family. The phosphorylation of Ser612 and Ser636/639 is mediated by the PKC and mTOR pathways, respectively and phosphorylation at Ser1101 is mediated by PKCtheta, resulting in an inhibition of insulin signaling in the cell, suggesting a potential mechanism for insulin resistance in some models of obesity.

Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1-2ug/ml
Immunohistochemistry: 2ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
PabAffinity Purified
100ug-20CBlue IceHumanRabbit
Synthetic peptide corresponding to 16aa near the center of human IRS-1.
Purified by immunoaffinity chromatography.
Supplied as a liquid in PBS, 0.02% sodium azide.
Recognizes human IRS-1. Species crossreactivity: mouse
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Giovannone, B., et al., (2000), "Insulin receptor substrate (IRS) transduction system: distinct and overlapping signaling potential", Diabetes Metab. Res. Rev., 16: 434-441, 2. Waters, S.B., et al., (1996), "Insulin receptor substrate 1 and 2 (IRS1 and IRS2): what a tangled web we weave", Trends in Cell Biol., 6: 1-4,, 3. Ozes, O.N., et al., (2001), "A phosphatidylinositol 3-kinase/Akt/mTOR pathway mediates and PTEN antagonizes tumor necrosis factor inhibition of insulin signaling through insulin receptor substrate-1", Proc. Natl. Acad. Sci., USA, 98: 4640-4645, 4. Shamji, A.F., et al., (2003), "Integration of growth factor and nutrient signaling: implications for cancer biology", Mol. Cell, 12: 271-280.