Technical Data
I8700-07A
IRS-1 (Insulin Receptor Substrate 1)
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Technical Data |
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I8700-07A |
IRS-1 (Insulin Receptor Substrate 1) |
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Description: IRS-1 (Insulin receptor substrate-1) is phosphorylated in response to stimulation of cells by insulin. Upon ligand-induced phosphorylation, IRS-1 immediately associates with a series of SH2 domain-containing signaling intermediates such as PI3-kinase, GRB2, Syp and Nck. The level of diversity in structure and function of the SH2-containing proteins which binds activated IRS-1 is important for responsiveness to insulin. IRS phosphorylation elicits the growth and metablic effects of insulin growth factors and insulin by allowing glucose uptake, glycogen synthesis and mitogenic activity. IRS-1 migrates between 170 and 185kD and is expressed in most cell types. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications have not been tested. Recommended Dilutions: Western Blot: 1-2 ug/ml Optimal dilutions to be determined by the researcher. Control Peptide: I8700-07A1 Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
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