Technical Data
I8700-07A
IRS-1 (Insulin Receptor Substrate 1)
Description:
IRS-1 (Insulin receptor substrate-1) is phosphorylated in response to stimulation of cells by insulin. Upon ligand-induced phosphorylation, IRS-1 immediately associates with a series of SH2 domain-containing signaling intermediates such as PI3-kinase, GRB2, Syp and Nck. The level of diversity in structure and function of the SH2-containing proteins which binds activated IRS-1 is important for responsiveness to insulin. IRS phosphorylation elicits the growth and metablic effects of insulin growth factors and insulin by allowing glucose uptake, glycogen synthesis and mitogenic activity. IRS-1 migrates between 170 and 185kD and is expressed in most cell types.

Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications have not been tested.

Recommended Dilutions:
Western Blot: 1-2 ug/ml
Optimal dilutions to be determined by the researcher.

Control Peptide: I8700-07A1

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanRabbit
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide mapping to the C-terminus of human IRS-1
Purity:
Purified by peptide affinitiy chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 1% BSA, 0.02% thimerosal, 50% glycerol.
Specificity:
Recognizes human IRS-1 at ~175kD. It does not cross-react with IRS-2, -3, and -4. Species crossreactivity: mouse, rat, monkey, horse, dog, pig
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.