Technical Data

Media

Iscove’s Modified Dulbecco’s w/L-Glutamine, 25mM HEPES (Powder)

I8750

Cell Culture Grade

Storage RT/4°C Shipping RT

Components shown as g/liter


Inorganic Salts:
Calcium Chloride•2H2O	0.219
Magnesium Sulfate	0.09767
Potassium Chloride	0.33
Potassium Nitrate	0.000076
Sodium Chloride	4.505
SodiumSelenite	0.000017
Sodium Phosphate Monobasic	0.109

Amino Acids:
L-Alanine	0.025
L-Arginine	0.084
L-Aspartic Acid	0.03
L-Asparagine•H2O	0.0284
L-Cystine•2HCI	0.09124
L-Glutamic Acid	0.075
L-Glutamine	0.584
Glycine	0.03
L-Histidine•HCl•H2O	0.042
L-Isoleucine	0.105
L-Leucine	0.105
L-Lysine•HCl	0.146
L-Methionine	0.03
L-Phenylalanine	0.066
L-Proline	0.04
L-Serine	0.042
L-Threonine	0.095
L-Tryptophan	0.016
L-Tyrosine•2Na•2H2O	0.10379
L-Valine	0.094

Vitamins:
D-Biotin	0.000013
Choline Chloride	0.004
Folic Acid	0.004
myo-Inositol	0.0072
Niacinamide	0.004
D-Pantothenic Acid•Ca	0.004
Pyridoxal•HCl	0.004
Riboflavin	0.0004
Thiamine•HCl	0.004
VitaminB12	0.000013

Other:
D-Glucose	4.5
HEPES	5.958
Pyruvic Acid, Sodium	0.11
Phenol Red, Sodium	0.016

Total:	17.7g/liter

Directions per Liter: Dissolve 17.7g in 800-900ml of ddH2O stirring gently until completely solubilized. Add 3.02g/L sodium bicarbonate. Adjust pH of the medium to the desired level. Add additional water to bring the solution to1L Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.

Storage and Stability: Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.

Appearance: Light pink to light orange, homogeneous, free flowing powder

Solubility: Light yellow, clear and complete

pH: As reported

Endotoxin: ~10Eu/mg

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

1. Iscove, N.N and Melchers, F. J. Exp. Medicine. 147, 923-933. (1978). 2. Iscove, N.N., Guilbert, L.J. and Weyman, C. Cell Research. 126, 121-126 (1980).