Technical Data
Media
Iscove’s Modified Dulbecco’s w/L-Glutamine, 25mM HEPES, w/o Phenol Red (Powder)
I8750-01
Cell Culture Grade
Storage RT/4°C    Shipping RT
Components shown as g/liter
Inorganic Salts:
Calcium Chloride•2H2O0.219
Magnesium Sulfate0.09767
Potassium Chloride0.330
Potassium Nitrate0.000076
Sodium Chloride4.505
Sodium Phosphate Monobasic0.109
Sodium Selenite0.000017
Amino Acids:
L-Alanine0.025
L-Arginine0.084
L-Asparagine•H2O0.0284
L-Aspartic Acid0.030
L-Cystine•2HCI0.09124
L-Glutamic Acid0.075
L-Glutamine0.584
Glycine0.030
L-Histidine•HCl•H2O0.042
L-Isoleucine0.105
L-Leucine0.105
L-Lysine•HCl0.146
L-Methionine0.030
L-Phenylalanine0.066
L-Proline0.040
L-Serine0.042
L-Threonine0.095
L-Tryptophan0.016
L-Tyrosine•2Na•2H2O0.10379
L-Valine0.094
Vitamins:
D-Biotin0.000013
Choline Chloride0.004
Vitamin B120.000013
Folic Acid0.004
myo-Inositol0.0072
Niacinamide0.004
D-Pantothenic Acid•Ca0.004
Pyridoxal•HCl0.004
Riboflavin0.0004
Thiamine•HCl0.004
Other:
D-Glucose4.500
HEPES5.958
Pyruvic Acid, Sodium0.110
Phenol Red, SodiumAbsent
Total:17.7g/liter
In 1976, Guilbert and Iscove demonstrated that precursor cells of erythrocytes and macrophages
could be cultured in a reduced-serum medium supplemented with albumin, transferrin, lecithin, and selenium. Iscove's medium is a modification of Dulbecco's Modified Eagle's Medium (DME)
containing selenium, additional amino acids and vitamins, sodium pyruvate, HEPES buffer, and
potassium nitrate instead of ferric nitrate.


Directions per Liter: Dissolve 17.7g in 800-900ml of ddH2O stirring gently until completely solubilized. Add 3.02g/L sodium bicarbonate. Adjust pH of the medium to the desired level. Add additional water to bring the solution to1L Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.

Storage and Stability: Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.

Appearance: Off-white to light yellow, homogeneous, free flowing powder.

Solubility: Colorless to light yellow, clear, complete

pH: As Reported

Endotoxin: 1EU/ml

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

1. Iscove, N.N and Melchers, F. J. Exp. Medicine. 147, 923-933. (1978).
2. Iscove, N.N., Guilbert, L.J. and Weyman, C. Cell Research. 126, 121-126 (1980).