Technical Data
Media
Iscove’s Modified Dulbecco’s w/o L-Glutamine, HEPES, Glucose, Phenol Red, Inorganic Salts (Powder)
I8750-02
Cell Culture Grade
Storage RT/4°C    Shipping RT
Directions per Liter:
Dissolve 1.39g in 800-900ml of ddH2O stirring gently until completely solubilized. Add salts, buffers as needed. Add 3.02g/L sodium bicarbonate. Adjust pH of the medium to the desired level. Add additional water to bring the solution to1L Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.
Storage and Stability:
Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.
Components shown as g/liter
Inorganic Salts:
Calcium Chloride•2H2OAbsent
Magnesium SulfateAbsent
Potassium ChlorideAbsent
Potassium NitrateAbsent
Sodium ChlorideAbsent
Sodium SeleniteAbsent
Sodium Phosphate MonobasicAbsent
Amino Acids:
L-Alanine0.025
L-Arginine0.084
L-Asparagine•H2O0.0284
L-Aspartic Acid0.03
L-Cystine•2HCI0.09124
L-Glutamic Acid0.075
L-GlutamineAbsent
Glycine0.03
L-Histidine•HCl•H2O0.042
L-Isoleucine0.105
L-Leucine0.105
L-Lysine•HCl0.146
L-Methionine0.03
L-Phenylalanine0.066
L-Proline0.04
L-Serine0.042
L-Threonine0.095
L-Tryptophan0.016
L-Tyrosine•2Na•2H2O0.10379
L-Valine0.094
Vitamins:
D-Biotin0.000013
Choline Chloride0.004
Vitamin B120.000013
Folic Acid0.004
myo-Inositol0.0072
Niacinamide0.004
D-Pantothenic Acid•Ca0.004
Pyridoxal•HCl0.004
Riboflavin0.0004
Thiamine•HCl0.004
Other:
D-GlucoseAbsent
HEPESAbsent
Pyruvic Acid, Sodium0.11
Phenol Red, SodiumAbsent
Total:1.39g/liter



Directions per Liter: Dissolve 1.39g in 800-900ml of ddH2O stirring gently until completely solubilized. Add salts, buffers as needed. Add 3.02g/L sodium bicarbonate. Adjust pH of the medium to the desired level. Add additional water to bring the solution to1L Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.

Storage and Stability: Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

1. Iscove, N.N and Melchers, F. J. Exp. Medicine. 147, 923-933. (1978).
2. Iscove, N.N., Guilbert, L.J. and Weyman, C. Cell Research. 126, 121-126 (1980).