Technical Data
Media
Iscove’s Modified Dulbecco’s w/o D-Glucose, L-Glutamic Acid, L-Glutamine, Na Pyruvate, Thiamine HCl w/25mM HEPES (Powder)
I8750-06
Cell Culture Grade
Storage RT/4°C    Shipping RT
Components shown as g/liter
Inorganic Salts:
Calcium Chloride•2H2O0.219
Magnesium Sulfate0.09767
Potassium Chloride0.33
Potassium Nitrate0.000076
Sodium Chloride4.505
Sodium Phosphate Monobasic0.109
Sodium Selenite0.000017
Amino Acids:
L-Alanine0.025
L-Arginine0.084
L-Asparagine•H2O0.0284
L-Aspartic Acid0.03
L-Cystine•2HCI0.09124
Glycine0.03
L-Histidine•HCl•H2O0.042
L-Isoleucine0.105
L-Leucine0.105
L-Lysine•HCl0.146
L-Methionine0.03
L-Phenylalanine0.066
L-Proline0.04
L-Serine0.042
L-Threonine0.095
L-Tryptophan0.016
L-Tyrosine•2Na•2H2O0.10379
L-Valine0.094
Vitamins:
D-Biotin0.000013
Choline Chloride0.004
Vitamin B120.000013
Folic Acid0.004
myo-Inositol0.0072
Niacinamide0.004
D-Pantothenic Acid•Ca0.004
Pyridoxal•HCl0.004
Riboflavin0.0004
Other:
HEPES5.958
Phenol Red, Sodium0.016
Total:12.4g/liter
Appearance:
Light pink to light orange, homogeneous, free flowing powder

Solubility:
Light yellow to yellow, clear, complete

pH:
As Reported

Endotoxin:
1EU/ml


Directions per Liter: Dissolve 12.4g in 800-900ml of ddH2O stirring gently until completely solubilized. Add 3.02g/L sodium bicarbonate. Adjust pH of the medium to the desired level. Add additional water to bring the solution to1L Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.

Storage and Stability: Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

1. Iscove, N.N and Melchers, F. J. Exp. Medicine. 147, 923-933. (1978). 2. Iscove, N.N., Guilbert, L.J. and Weyman, C. Cell Research. 126, 121-126 (1980).