Technical Data
Media
Iscove’s Modified Dulbecco’s w/L-Glutamine w/o a-Thioglycerol, 2ME (Special Modified) (Powder)
I8751
Cell Culture Grade
Storage RT    Shipping RT
Components shown as mg/liter
Inorganic Salts:
Calcium Chloride218.56
Potassium Chloride330
Potassium Nitrate0.076
Magnesium Sulfate97.67
Sodium Chloride4505
Sodium Phosphate Monobasic108.69
Sodium Selenite0.017
Amino Acids:
L-Alanine25
L-Arginine84
L-Asparagine28
L-Aspartic Acid30
L-Cystine•2HCI91.24
L-Glutamic Acid20
L-Glutamine75
Glycine584
L-Histidine•HCl*H2O42
L-Isoleucine104.8
L-Leucine104.8
L-Lysine•HCI14.62
L-Methionine30
L-Phenylalanine66
L-Proline40
L-Serine42
L-Threonine95.2
L-Tryptophan16
L-Tyrosine•2Na103.79
L-Valine93.6
Vitamins:
Biotin0.013
D-Pantothenic Acid•Ca4
Choline Chloride4
Folic Acid4
i-Inositol7
Nicotinamide4
Pyridoxal•HCI4
Riboflavin0.4
Thiamine•HCI4
Vitamin B120.013
Other:
D-Glucose4500
Phenol Red15.34
Sodium Pyruvate110
Total:11.6g



Directions per Liter Dissolve 11.6 grams per liter of distilled/deionized (DDI) water, heating with stirring until completely solubilized, adjusting pH as necessary. If required, add sodium bicarbonate with stirring. Sterilize with 0.2µ membrane filter. Aliquot into sterile containers.

Storage Store at 0-5ºC (store unopened media at 2-30°C). Opened bottles should be capped tightly and kept in a dark, low humidity environment.


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

1. Iscove, N.N and Melchers, F. J. Exp. Medicine 147:923-933 (1978) 2. Iscove, N.N., Guilbert, L.J. and Weyman, C. Cell Research 126:121-126 (1980)