Technical Data

Media

Iscove’s Modified Dulbecco’s w/L-Glutamine w/o a-Thioglycerol, 2ME (Special Modified) (Powder)

I8751

Cell Culture Grade

Storage RT Shipping RT

Components shown as mg/liter


Inorganic Salts:
Calcium Chloride	218.56
Potassium Chloride	330
Potassium Nitrate	0.076
Magnesium Sulfate	97.67
Sodium Chloride	4505
Sodium Phosphate Monobasic	108.69
Sodium Selenite	0.017

Amino Acids:
L-Alanine	25
L-Arginine	84
L-Asparagine	28
L-Aspartic Acid	30
L-Cystine•2HCI	91.24
L-Glutamic Acid	20
L-Glutamine	75
Glycine	584
L-Histidine•HCl*H2O	42
L-Isoleucine	104.8
L-Leucine	104.8
L-Lysine•HCI	14.62
L-Methionine	30
L-Phenylalanine	66
L-Proline	40
L-Serine	42
L-Threonine	95.2
L-Tryptophan	16
L-Tyrosine•2Na	103.79
L-Valine	93.6

Vitamins:
Biotin	0.013
D-Pantothenic Acid•Ca	4
Choline Chloride	4
Folic Acid	4
i-Inositol	7
Nicotinamide	4
Pyridoxal•HCI	4
Riboflavin	0.4
Thiamine•HCI	4
Vitamin B12	0.013

Other:
D-Glucose	4500
Phenol Red	15.34
Sodium Pyruvate	110

Total:	11.6g

Directions per Liter Dissolve 11.6 grams per liter of distilled/deionized (DDI) water, heating with stirring until completely solubilized, adjusting pH as necessary. If required, add sodium bicarbonate with stirring. Sterilize with 0.2µ membrane filter. Aliquot into sterile containers.

Storage Store at 0-5ºC (store unopened media at 2-30°C). Opened bottles should be capped tightly and kept in a dark, low humidity environment.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

1. Iscove, N.N and Melchers, F. J. Exp. Medicine 147:923-933 (1978) 2. Iscove, N.N., Guilbert, L.J. and Weyman, C. Cell Research 126:121-126 (1980)