Technical Data
Media
Iscove’s Modified Dulbecco’s w/L-Glutamine w/o a-Thioglycerol, 2ME (Powder)
I8751-05
Cell Culture Grade
Storage RT    Shipping RT
Components shown as mg/liter
Inorganic Salts:
Calcium Chloride•2H2O218.56
Potassium Chloride330.00
Potassium Nitrate0.076
Magnesium Sulfate, anhy97.67
Sodium Chloride4505.00
Sodium Phosphate Monobasic•H2O125.00
Sodium Selenite0.017
Amino Acids:
L-Alanine25.00
L-Arginine•HCl84.00
L-Asparagine, anhy.28.00
L-Aspartic Acid30.00
L-Cystine•2HCI91.20
L-Glutamic Acid75.00
L-Glutamine584.00
Glycine30.00
L-Histidine•HCl•H2O42.00
L-Isoleucine104.80
L-Leucine104.80
L-Lysine•HCI146.00
L-Methionine30.00
L-Phenylalanine66.00
L-Proline40.00
L-Serine42.00
L-Threonine95.20
L-Tryptophan16.00
L-Tyrosine•2Na103.79
L-Valine93.60
Vitamins:
Biotin0.013
D-Pantothenic Acid•Ca4.00
Choline Chloride4.00
Folic Acid4.00
i-Inositol7.20
Nicotinamide4.00
Pyridoxal•HCI4.00
Riboflavin0.40
Thiamine•HCI4.00
Vitamin B120.013
Other:
D-Glucose4500.00
Phenol Red15.00
Sodium Pyruvate 110.00
Total:11.6g/Liter
Appearance: Off white free flowing powder

Solubility (11.6g/L): Clear, yellow and complete

pH: 4.9 ± 0.3


Directions per Liter Dissolve 11.6 grams per liter of distilled/deionized (DDI) water, heating with stirring until completely solubilized, adjusting pH as necessary. If required, add sodium bicarbonate with stirring. Sterilize with 0.2um membrane filter. Aliquot into sterile containers.

Storage Store at 0-5ºC (store unopened media at 2-30°C). Opened bottles should be capped tightly and kept in a dark, low humidity environment.


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

1. Iscove, N.N and Melchers, F. J. Exp. Medicine 147:923-933 (1978)
2. Iscove, N.N., Guilbert, L.J. and Weyman, C. Cell Research 126:121-126 (1980)