		Technical Data
I9039-98A	ICMT, CT (Protein-S-isoprenylcysteine O-methyltransferase, Isoprenylcysteine Carboxylmethyltransferase, Prenylcysteine Carboxyl Methyltransferase, pcCMT, Prenylated Protein Carboxyl Methyltransferase, PPMT, ICMT, PCCMT)

Description:
This gene encodes the third of three enzymes that posttranslationally modify isoprenylated C-terminal cysteine residues in certain proteins and target those proteins to the cell membrane. This enzyme localizes to the endoplasmic reticulum. Alternative splicing results in two transcript variants encoding different isoforms.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:1,000
Western Blot: 1:100-1:500
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab	IgG		Affinity Purified

Size		Storage	Shipping	Source	Host
200ul		-20°C	Blue Ice	Human	Rabbit

Concentration:

~0.25mg/ml

Immunogen:

Synthetic peptide selected from the C-terminal region of human ICMT (KLH).

Purity:

Purified by Protein G affinity chromatography.

Form

Supplied as a liquid in PBS, 0.09% sodium azide.

Specificity:

Recognizes human ICMT.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1.Bergo, M.O., et al., J. Clin. Invest. 113(4):539-550 (2004). 2.Ahmad, M., et al., Arterioscler. Thromb. Vasc. Biol. 22(5):759-764 (2002). 3.Van Dessel, G.A., et al., Int. J. Biochem. Cell Biol. 34(5):477-486 (2002). 4.Dai, Q., et al., J. Biol. Chem. 273(24):15030-15034 (1998).