Technical Data
J0400-01K
Jagged 1 (JAG1, AGS, AHD, AWS, HJ1, JAGL1, Alagille Syndrome Protein, CD339, Headturner, Htu, MGC104644, Serrate-1, Ser-1, Slalom)
Description:
Jagged 1 is a 180kD type I transmembrane glycoprotein and member of the Delta-Serrate-Lag-2 (DSL) family of ligands that activate LIN12/Notch proteins. Human Jagged 1 is synthesized as a 1218aa precursor that contains a 33aa signal sequence, a 1034aa extracellular domain (ECD), a 26aa transmembrane segment, and a 125aa cytoplasmic region. The ECD contains a DSL domain (aa185-229), a cysteine-rich region, 15 EGF-like repeats, of which many bind calcium, and nine potential sites for N-linked glycosylation. Mature human Jagged 1 shares 97-96aa identity with mature mouse and rat Jagged 1, respectively. Jagged 1 is widely expressed in adult and fetal tissues. Jagged-Notch signaling specifies cell fate, regulates pattern formation, defines boundaries between different cell types, and modulates cell proliferation and differentiation, especially during hematopoiesis, myogenesis, neurogenesis, and development of vasculature. Mutations in human Jagged 1 are the cause of Alagille syndrome, an autosomal-dominant disorder characterized by intrahepatic cholestasis and abnormalities of heart, eye, vertebrae, as well as characteristic facial appearance.

Applications:
Suitable for use in Flow Cytometry. Other applications not tested.

Recommended Dilutions:
Flow Cytometry: 2.5ug labels 10e6 cells. Using K562 human chronic myelogenous leukemia cell line, bEnd.3 mouse endothelioma cell line and rat cortical stem cells.
Optimal dilutions to be determined by the researcher.

Hybridoma:
NSO myeloma cells with B cells from Balb/c mice.

Storage and Stability:
Lyophilized powder may be stored at -20░C. Stable for 12 months at -20░C after receipt. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20░C. Reconstituted product is stable for 12 months at -20░C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG2b11n115Affinity Purified
SizeStorageShippingSourceHost
500ug-20░CBlue IceHumanMouse
Concentration:
~0.5mg/ml (after reconstitution)
Immunogen:
Recombinant protein corresponding to aa32-296 from human Jagged 1 expressed in NSO cells (P78504).
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 1ml sterile PBS.
Specificity:
Recognizes human Jagged 1. Does not recognize recombinant human Jagged 2. Stains human Jagged 1 transfectants but not the parental cell line or human Jagged 2 transfectants in Flow Cytometry.áSpecies Crossreactivity: mouse and rat
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Sainson, R.C. and A.L. Harris (2008) Angiogenesis 11:41. 2. Cordle, J. et al. (2008) Nat. Struct. Mol. Biol. 15:849. 3. Artavamis-Tsokanas, S. et al. (1999) Science 284:770. 4. Lai, E.C. (2004) Development 131:965. 5. Milner, L.A. et al. (1994) Blood 83:2057. 6. Nyfeler, Y. et al. (2005) EMBO J. 24:3504 7. Linheng, L. et al. (1998) Immunity 8:43. 8. Iso, T. et al. (2003) Arterioscler. Thromb. Vasc. Biol. 23:543. 9. Oda, T. et al. (1997) Nat. Genet. 16:235. 10. Oda, T. et al. (1997) Genomics 43:376.