Technical Data
J Chain
J Chain serves to link two monomer units of either IgM or IgA. In the case of IgM, the J chain-joined dimer is a nucleating unit for the IgM pentamer, and in the case of IgA it induces larger polymers. It also help to bind these immunoglobulins to secretory component.

Suitable for use in Immunohistochemistry, ELISA, Immunoprecipitation and Western Blot. Other applications have not been tested.

Recommended Dilution:
Immunohistochemistry (Frozen): 1:200
ELISA: 20ug/ml
Immunoprecipitation: 20ug/ml
Optimal working dilutions to be determined by researcher.

NS1 myeloma cells with spleen cells from Balb/c mice.

Recommended Secondary Antibodies:
I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo
I1904-06H IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo
I1904-14C: IgG, Fc (HRP) Pab Gt xMo
I1904-19C: IgG, F(ab)2 (HRP) Pab Gt xMo

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
MabIgG13H2560Affinity Purified
200ug-20CBlue IceHumanMouse
Human J Chain.
Purified by Protein A affinity chromatography.
Supplied as a liquid in PBS, pH 7.0, 0.09% sodium azide.
Recognizes human J chain as tested by ELISA using purified human Ig fragments as the solid phase target antigen. The antibody does not detect J chain in intact IgM in ELISA assays due to steric hindrance. In Western Blot techniques the antibody detects J chain in IgM only in reducing conditions.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1.Arnold, J N et al., J Biol. Chem 280: 29080-29087