Technical Data
J2500-10H
JNK1/2, phosphorylated (Thr183, Tyr185), Human (c-Jun NH2-terminal Kinase1/2, SAPK alpha/gamma) BioAssay™ ELISA Kit
96Tests
Kits and Assays Storage: 4°C/-70°CShipping: Blue Ice
Sensitivity:
<0.8units/ml

Range:
1.6-100units/ml

Incubation Time:
4 hours

Simple:
Pre-coated strip-well plates. Liquid stable chromogen and stop reagents.

Sample size:
10ul

Economical:
One 96-well plate, plus reagents for 96 determinations. Reagents are stable for multiple runs.

Sample type:
Cell extracts/lysates, buffered solutions

Precision:
Intra-assay: <10 %.
Inter-assay: <10 %

Kit Components:
*J2500-10H1 Standard 2x1vial
J2500-10H2 Standard Diluent Buffer 1x25ml Contains 15 mM sodium azide
J2500-10H3 Microtiter Plate 1x96 wells
J2500-10H4 JNK1/2 (pTpY183/185) Pab 1x11ml Contains 15 mM sodium azide;J2500-10H 5 IgG Pab (HRP) (100x) 1x125ul Contains 3.3 mM thymol
J2500-10H6 HRP Diluent 1x25ml Contains 3.3 mM thymol
J2500-10H7 Wash Buffer (25x) 1x100ml
J2500-10H78Tetramethylbenzidine (TMB) 1x25ml
J2500-10H9 Stop Solution 1x25ml

Storage and Stability:
Store *J2500-10H1 Standard powder at 4°C liquid at -70°C. Store other components at 4°C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to opening.
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Dong, C., et al. (2001) Signaling by the JNK group of MAP kinases. C-jun N-terminal Kinase. J. Clin. Immunol. 21:253-7. 2. Kuhl, M. (2002) Non-canonical Wnt signaling in Xenopus: regulation of axis formation and gastrulation. Semin. Cell Dev. Biol. 13:243-249. 3. Leppa, S., Bohmann, D. (1999) Diverse functions of JNK signaling and c-Jun in stress response and apoptosis. Oncogene 18:6158-6162. 4. Weston, C.R., et al. (2002) Signal transduction. MAP kinase signaling specificity. Science 296:2345-2347. 5. Davis, R.J. Signal transduction by the JNK group of MAP kinases. Cell 103:239-52. 6. Tournier, C., et al. (2001) MKK7 is an essential component of the JNK signal transduction pathway activated by proinflammatory cytokines. Genes Dev. 15:1419-1426. 7. Chen, N., et al. (2001) Suppression of skin tumorigenesis in c-Jun NH(2)-terminal kinase-2-deficient mice. Cancer Res. 61:3908-3912. 8. Tournier, C., et al. (2000) Requirement of JNK for stress-induced activation of the cytochrome c-mediated death pathway. Science 88:870-874. 9. Levresse, V., et al. (2002) Regulation of Platinum-Compound Cytotoxicity by the c-Jun N-Terminal Kinase and c-Jun Signaling Pathway in Small-Cell Lung Cancer Cells. Mol. Pharmacol. 262:689-697. 10. Bogoyevitch, M.A., et al. (1995) Cellular stresses differentially activate c-Jun N-terminal protein kinases and extracellular signal-regulated protein kinases in cultured ventricular myocytes. J. Biol. Chem. 270:29710-29717.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.