Technical Data
JNK2 alpha2, unactive, Recombinant, Human (c-Jun N-terminal Kinase 2, JNK2, JNK2alpha, JNK2A, JNK-55, Mitogen-activated Protein Kinase 9, MAP Kinase 9, MAPK 9, MAPK9, PRKM9, p54a, p54aSAPK, Stress-activated Protein Kinase 1a, SAPK1a, SAPK, Stress-activate
Molecular Biology Storage: -20CShipping: Blue Ice
Jun Kinases (JNK), also called stress-activated protein (SAP) kinases, are proline-directed, serine/threonine-specific kinases that require phosphorylation on tyrosine for phosphotransferase activity. JNK was originally shown to phosphorylate microtubule-associated protein-2, but c-Jun and ATF2 appears to be an important physiological target of this kinase. Phosphorylation of Thr69 and Thr71 of ATF2 by JNK is important for the transcriptional activity of ATF2. cDNA cloning studies with rat and human libraries have shown that there are several isoforms of the JNK family. Many stimuli activate JNK including heat shock, hyperosmotic shock, UV irradiation, exposure to protein synthesis inhibitors such as cycloheximide and anisomycin, and treatment with proinflammatory cytokines such as tumor necrosis factor-a.

Recombinant full-length human JNK2a2/SAPK1a with an N-terminal His-tag, expressed in Sf21 cells. Accession number L31951

JNK2a2/SAPK1a Kinase Assay: 2mg of unactive JNK2a2/SAPK1a was incubated with MKK7/SKK4. Activation of JNK2a2/SAPK1a was assessed by phosphorylation of ATF2 substrate.

Specific Activity: 80u/mg when maximally activated. One unit of JNK2a2/SAPK1a activity is defined as 1nmol phosphate incorporated into GST-ATF2 per minute.

Storage and Stability: Stable for 6 months at -20C from date of shipment.

Molecular Weight:
Source: Recombinant human

Purity: 90%. Purified using Ni-NTA agarose. Mr ~51kD (SDS-PAGE and Coomassie blue staining)

Form: 100ug enzyme in 50ul of 50mM Tris-HCl, pH 7.5, 150mM NaCl, 0.1mM EGTA, 0.1% 2-mercaptoethanol, 0.02% Brij-35, 50% glycerol.

Intended for research use only, not for use in human, therapeutic or diagnostic applications.

1. Gupta, S., et al., EMBO J. 15: 27602770, 1996. 2. Gupta, S., et al., Science 267: 389393, 1995. 3. Lawler, S., et al., FEBS Lett 414: 153158, 1997.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.