Technical Data
J7876-47
Jumonji Domain Containing 2C (JMJD2C, JMJD2, Gene Amplified in Squamous Cell Carcinoma 1, GASC1, JmjC Domain Containing Histone Demethylation Protein 3C, JHDM3C, KDM4C)
Description:
Jumonji Domain Containing 2C is a member of the Jumonji domain 2 (JMJD2) family and encodes a protein with one JmjC domain, one JmjN domain, two PHD-type zinc fingers, and two Tudor domains. This nuclear protein functions as a trimethylation-specific demethylase, converting specific trimethylated histone residues to the dimethylated form. Chromosomal aberrations and increased transcriptional expression of this gene are associated with esophageal squamous cell carcinoma.

Applications:
Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested.

Recommended Dilutions:
Western Blot: 1:2000-1:10,000
Immunoprecipitation: 1:2000-1:10,000
Optimal dilutions to be determined by the researcher.

Positive Control:
HeLa whole cell extract

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ul-20°CBlue IceHumanRabbit
Concentration:
~1mg/ml
Immunogen:
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in Tris-citrate/phosphate buffer, pH 7.5, 0.09% sodium azide.
Specificity:
Recognizes human Jumonji Domain Containing 2C protein. The epitope recognized maps to a region between aa475-525 of human Jumonji Domain Containing 2C protein (NP_055876.2).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Liu, G., et al., Oncogene 28(50): 4491-4500 (2009).