Technical Data
Junctional Adhesion Molecule 1 (JAM-1, CD321, F11 Receptor, F11R, Junctional Adhesion Molecule A, JAMA, JCAM, KAT, Platelet Adhesion Molecule 1, PAM1, Platelet F11 Receptor, UNQ264/PRO301)
The junctional adhesion molecule-1 (JAM-1) is an integral membrane protein that co-localizes with tight junction molecules occludin, ZO-1, and cingulin.1 JAM-1 has been characterized as a member of the immunoglobulin (Ig) superfamily and is composed of an extracellular domain, a single-pass transmembrane region, and a short cytoplasmic tail.2 JAM-1 is localized at tight junctions in endothelial and epithelial cells and its sub-cellular distribution is modulated by inflammatory cytokines.2 JAM-1 is also expressed on circulating leukocytes and platelets as well as in lymphoid.2 Most claudins as well as JAM-1 end with the amino acid Val at their C-terminal ends, suggesting that these COOH termini directly bind to PDZ domains.3 JAM-1 appears to be a single copy gene, which is constitutively expressed on various tissues and cells, and may be involved in cell-to-cell adhesion through homophilic interaction.4 JAM-1 was detected in human intestine cell lines T84, CaCO2 and HT29, in the lung cell line A 549, and the liver cell line HepG2.5

Suitable for use in Western Blotting. Other applications not tested.

Recommended Dilution:
Western Blotting: 13ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
PabIgGAffinity Purified
100ug-20CBlue IceHumanRabbit
Synthetic peptide corresponding to the C-terminal of human JAM-1 protein
Purified by immunoaffinity chromatography.
Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.
Speci c for human ~3641kD JAM-1 protein. Reactivity has been con rmed with Caco-2 colorectal adenocarcinoma cell lysates.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Bazzoni G, et al. J Biol Chem, 275:20520-20526, 2000.
2. Bazzoni G, et al. J Biol Chem, 275:30970-30976, 2000.
3. Itoh M, et al. J Cell Biol, 154:491-497, 2001.
4. Naik UP, et al. J Cell Sci, 114: 539-547, 2001.
5. Liu Y, et al. J Cell Sci, 113: 2363-2374, 2000.