Technical Data
J8009-01
Junctional Adhesion Molecule 1 (JAM-1, CD321, F11 Receptor, F11R, Junctional Adhesion Molecule A, JAMA, JCAM, KAT, Platelet Adhesion Molecule 1, PAM1, Platelet F11 Receptor, UNQ264/PRO301)
Description:
Junctional adhesion molecule-1 (JAM-1) also known as the mouse platelet F11-Receptor (F11R) is a cell adhesion molecule (CAM). JAM-1 is a member of the immunoglobulin superfamily found on the surface of mouse platelets and at intercellular junctions of endothelial cells and epithelial cells. JAM-1 belongs together with JAM-2 and JAM-3 to a family of adhesion proteins with a V-C2 immunoglobulin domain organization. JAM plays an important role in tight junctions where it is involved in cell-to-cell adhesion through homophilic interaction. It codistributes with other tight junction components as ZO-1, 7H6 antigen, cingulin and occludin. In human JAM-1 plays a role in platelet aggregation, secretion, adhesion and spreading.

Applications:
Suitable for use in ELISA, Flow Cytometry, Western Blot, Immunoprecipitation and Immunofluorescence.

Recommended Dilution:
Flow Cytometry: 1:50
Western Blot: 1:50
Immunofluorescence: Fixed cells.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG2a6A587Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceMouseRat
Concentration:
~0.1mg/ml
Immunogen:
Mouse JAM-1
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, 0.1% BSA, 0.02% sodium azide.
Specificity:
Recognizes mouse JAM-1.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Bazzoni, G., et al., J. Biol. Chem. 275: 20,520 (2000). 2. Martinez-Estrada, O., et al., Am. J. Physiol. Lung Cell Mol. Physiol. 288: L1081 (2005).