Technical Data
J8009-02
Junctional Adhesion Molecule 1 (JAM-1, CD321, F11 Receptor, F11R, Junctional Adhesion Molecule A, JAMA, JCAM, KAT, Platelet Adhesion Molecule 1, PAM1, Platelet F11 Receptor, UNQ264/PRO301)
Description:
Junctional adhesion molecule-1 (JAM-1) also known as the human platelet F11-Receptor (F11R) is a cell adhesion molecule (CAM). JAM-1 is a member of the immunoglobulin superfamily found on the surface of human platelets and at intercellular junctions of endothelial cells and epithelial cells. JAM-1 belongs together with JAM-2 and JAM-3 to a family of adhesion proteins with a V-C2 immunoglobulin domain organization. JAM plays an important role in tight junctions where it is involved in cell-to-cell adhesion through homophilic interaction. It codistributes with other tight junction components as ZO-1, 7H6 antigen, cingulin and occludin. JAM-1 plays a role in platelet aggregation, secretion, adhesion, spreading. In the platelet F11R/Jam-1 is a membrane protein involved in 2 distinct processes initiated on the platelet surface. Antibody-induced platelet aggregation and secretion both dependent on FcgammaRII and GPIIb/IIIa integrin, a process that may be involved in pathophysiological processes associated with certain thrombocytopenias. Antibody mediated platelet adhesion independent from FcgammaRII or fibrinogen receptor and that appears to play a role in physiological processes associated with platelet adhesion and aggregation. A physiological role for the F11R/Jam-1 protein was demonstrated by its phosphorylation after the stimulation of platelets by thrombin and collagen. A pathophysiological role for the F11R/Jam-1 was revealed by demonstrating the presence of F11R/Jam-1 antibodies in patients with thrombocytopenia. Adhesion of platelets through the F11R resulted in events characteristic of the action of cell adhesion molecules (CAMs). Recent data suggests a role for F11R/Jam-1 in the adhesion of platelets to cytokine-inflamed endothelial cells and thus in thrombosis and atherosclerosis induced in non-denuded blood vessels by inflammatory processes.

Applications:
Can be used for Flow Cytometry and Immunohistology on frozen sections or cell monolayers.

Recommended Dilution:
Flow Cytometry: 1:10
Immunohistology: 1:10
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20C or colder. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG16A588Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanMouse
Concentration:
~0.1mg/ml
Immunogen:
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, containing 0.02% sodium azide and 0.1% BSA.
Specificity:
Recognizes human JAM-1.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Martin-Padura, I et al; Junctional adhesion molecule, a novel member of the immunoglobulin superfamily that distributes at intercellular junctions and modulates monocyte transmigration. J Cell Biol 1998, 13: 117. 2. Bazzoni, G et al; Interaction of junctional adhesion molecule with the tight junction components ZO-1, cingulin, and occludin. J Biol Chem 2000, 275: 20,520.