		Technical Data
J8009-02A	Junctional Adhesion Molecule 1 (Junctional Adhesion Molecule A, JAM-A, JAM-1, Platelet Adhesion Molecule 1, PAM-1, CD321, Platelet F11 Receptor, F11R, JAM1, JCAM, F11R, UNQ264/PRO301) (APC)

Description:
The members of junctional adhesion molecules family are type I transmembrane receptors belonging to the immunoglobulin (Ig) superfamily. Human JAM-A, also known as platelet adhesion molecule 1 (PAM-1) and platelet F11 receptor, is predominantly expressed at intercellular junctions of both epithelial cells and endothelial cells. It is also expressed on circulating blood cells including neutrophils, monocytes, platelets, erythrocytes, and lymphocytes. JAM-A exhibits homophilic interactions to regulate tight junction assembly and modulate paracellular permeability. This homophilic interaction also mediates platelet aggregation and adhesion to endothelial cells and may play a role in thrombosis. The human and mouse proteins share ~67% aa sequence homology. Human JAM-A also shares ~35% and 32% aa sequence homology with human JAM-B and JAM-C.

Applications:
Suitable for use in Flow Cytometry. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Excitation: 620-650nm

Emission: 660-670nm

Storage and Stability:
May be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. Stable for 12 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer. APC is light sensitive.

Type	Isotype	Clone	Grade
Mab	IgG1	12K120	Affinity Purified

Size		Storage	Shipping	Source	Host
100 Tests		4°C Do Not Freeze	Blue Ice	Human	Mouse

Concentration:

Not determined

Immunogen:

Recombinant corresponding to human JAM-A expressed in NSO cell line.

Purity:

Purified by Protein A/G affinity chromatography.

Form

.Supplied as a liquid in saline, 0.5% BSA, 0.1% sodium azide. Labeled with Allophycocyanin (APC).

Specificity:

Recognizes human JAM-A.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Chavakis, T. et al. (2003) Thromb. Haemost. 89:13. 2. Aurrand-Lions, M. et al. (2001) Blood 98:3699. 3. Sobocka, M.B. et al. (2000) Blood 95:2600. 4. Martin-Padura, I. et al. (1998) J. Cell Biol. 142:117. 5. Williams, L.A. et. al. (1999) Mol. Immunol. 36:1175.