Technical Data
J8009-03
Junctional Adhesion Molecule 1 (JAM-1, CD321, F11 Receptor, F11R, Junctional Adhesion Molecule A, JAMA, JCAM, KAT, Platelet Adhesion Molecule 1, PAM1, Platelet F11 Receptor, UNQ264/PRO301)
Description:
Junctional adhesion molecule-1 (JAM-1) also known as the human platelet F11-Receptor (F11R) is a cell adhesion molecule (CAM). JAM-1 is a member of the immunoglobulin superfamily found on the surface of human platelets and at intercellular junctions of endothelial cells and epithelial cells. JAM-1 belongs together with JAM-2 and JAM-3 to a family of adhesion proteins with a V-C2 immunoglobulin domain organization. JAM plays an important role in tight junctions where it is involved in cell-to-cell adhesion through homophilic interaction. It codistributes with other tight junction components as ZO-1, 7H6 antigen, cingulin and occludin. JAM-1 plays a role in platelet aggregation, secretion, adhesion, spreading.

Applications:
Suitable for use in Flow Cytometry, Western Blot and Immunoprecipitation. Other applications have not been tested.

Recommended Dilution:
Flow Cytometry: 1:50
Western Blot: 1:50
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

TypeIsotypeCloneGrade
MabIgG16A589Affinity Purified
SizeStorageShippingSourceHost
100ug4C (-20C Glycerol)Blue IceHumanMouse
Concentration:
~0.1mg/ml
Immunogen:
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, containing 0.1% BSA.
Specificity:
Recognizes human JAM-1. Directly stimulates the aggregation and granule secretion of human platelets.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Kornecki, E et al;J Biol Chem 1990, 265: 10,042. 2. Walkowisk B et al; Thromb Res 1992, 68: 323. 3. Naik, U et al; . Biochem J 1995, 310: 155. 4. Wang, F et al; Biochem J 1995, 311: 401. 5. Sobocka, M et al; Blood 2000, 95: 2600. 6. Babinska, A et al;. Thromb Haemost 2002, 87: 712.