Technical Data
J8009-04A
Junctional Adhesion Molecule 1 (JAM-1, CD321, F11 Receptor, F11R, Junctional Adhesion Molecule A, JAMA, JCAM, KAT, Platelet Adhesion Molecule 1, PAM1, Platelet F11 Receptor, UNQ264/PRO301) (FITC)
Description:
JAM-A is a member of the immunoglobulin superfamily found on the surface of mouse platelets and at intercellular junctions of endothelial cells and epithelial cells. JAM-A belongs together with JAM-B (VE-JAM or JAM-3) and JAM-C (JAM-2) to a family of adhesion proteins with a V-C2 immunoglobulin domain organization. JAM plays an important role in tight junctions where it is involved in cell-to-cell adhesion through homophilic interaction. It codistributes with other tight junction components as ZO-1, 7H6 antigen, cingulin and occludin. In human JAM-A plays a role in platelet aggregation, secretion, adhesion and spreading.

Applications:
Suitable for use in Immunofluorescence, Flow Cytometry, Immunoassays, and Western Blot. Other applications not tested.

Recommended Dilution:
Immunofluorescence: 1:50
Flow Cytometry: 1:50
Western Blot: 1:50
Optimal dilutions to be determined by the researcher.

Storage and Stability:
For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. FITC conjugates are sensitive to light.
TypeIsotypeCloneGrade
MabIgG9G203Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceMouseRat
Concentration:
As reported
Immunogen:
Mouse JAM-A.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, 1% BSA, 0.02% sodium azide. Labeled with fluorescein isothiocyanate (FITC).
Specificity:
Recognizes junctional adhesion molecule-A (JAM-A), also known as JAM-1 and the mouse platelet F11-Receptor (F11R), is a cell adhesion molecule (CAM).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Bazzoni, G et al; Interaction of junctional adhesion molecule with the tight junction components ZO-1, cingulin, and occludin. J Biol Chem 2000, 275: 20520 2. Martinez-Estrada, O et al; Opposite effects of tumor necrosis factor and soluble fibronectin on junctional adhesion molecule-A in endothelial cells. Am J Physiol Lung Cell Mol Physiol 2005, 288: L1081