Technical Data
J8009-04E4
Junctional Adhesion Molecule 1 (JAM 1, JAM-1, JAM1, CD321, F11 Receptor, F11R, Junctional Adhesion Molecule A, JAMA, JCAM, KAT, Platelet Adhesion Molecule 1, PAM1, Platelet F11 Receptor, UNQ264/PRO301) (Biotin)
Description:
Applications:
Suitable for use in Flow Cytometry. Other applications not tested.

Recommended Dilution:
Flow Cytometry: Neat-1:5; 10ul labels 10e6 cells in 100ul
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG110B1403Affinity Purified
SizeStorageShippingSourceHost
100ug4C/-20C (No Glycerol)Blue IceMouseRat
Concentration:
~0.1mg/ml
Immunogen:
MTE1/MTE2 stromal cell lines.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 1% BSA, 0.09% sodium azide. Labeled with Biotin.
Specificity:
Recognizes murine CD321.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Malergue, F.et al. (1998) A novel immunoglobulin superfamily junctional molecule expressed by antigen presenting cells, endothelial cells and platelets. Mol. Immunol.35:1111-1119, 2. Aurrand-Lions, M. et al. (2001) Heterogeneity of endothelial junctions is reflected by differential expression and specific subcellular localization of the three JAM family members. Blood.98:3699-3707, 3. Ebnett, K.et al. (2000) Junctional Adhesion Molecule Interacts with the PDZ Domain-containing Proteins AF-6 and ZO-1. J. Biol. Chem.275:27979-27988