Technical Data
J8009-04F
Junctional Adhesion Molecule 1 (JAM 1, JAM-1, JAM1, CD321, F11 Receptor, F11R, Junctional Adhesion Molecule A, JAMA, JCAM, KAT, Platelet Adhesion Molecule 1, PAM1, Platelet F11 Receptor, UNQ264/PRO301)
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Technical Data |
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J8009-04F |
Junctional Adhesion Molecule 1 (JAM 1, JAM-1, JAM1, CD321, F11 Receptor, F11R, Junctional Adhesion Molecule A, JAMA, JCAM, KAT, Platelet Adhesion Molecule 1, PAM1, Platelet F11 Receptor, UNQ264/PRO301) |
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Description: JAM-1, cell surface antigen, originally identified by this antibody as the F11R antigen on platelets. JAM-1, also known as CD321, is important in intercellular adhesion between endothelial cell and epithelial cells, and is also a functional receptor on platelets. Applications: Suitable for use in Flow Cytometry, Immunoprecipitation, and Western Blot. Other applications not tested. Recommended Dilution: Flow Cytometry: 1:10-1:50; 10ul labels 10e6 cells in 100ul Western Blot: 1:100-1:1,000 Optimal dilutions to be determined by the researcher. Hybridoma: Sp2/0 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
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