Technical Data
J8009-04F
Junctional Adhesion Molecule 1 (JAM 1, JAM-1, JAM1, CD321, F11 Receptor, F11R, Junctional Adhesion Molecule A, JAMA, JCAM, KAT, Platelet Adhesion Molecule 1, PAM1, Platelet F11 Receptor, UNQ264/PRO301)
Description:
JAM-1, cell surface antigen, originally identified by this antibody as the F11R antigen on platelets.
JAM-1, also known as CD321, is important in intercellular adhesion between endothelial cell and epithelial cells, and is also a functional receptor on platelets.

Applications:
Suitable for use in Flow Cytometry, Immunoprecipitation, and Western Blot. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 1:10-1:50; 10ul labels 10e6 cells in 100ul
Western Blot: 1:100-1:1,000
Optimal dilutions to be determined by the researcher.

Hybridoma:
Sp2/0 myeloma cells with spleen cells from Balb/c mice.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG110B1402Purified
SizeStorageShippingSourceHost
200ug4C/-20C (No Glycerol)Blue IceHumanMouse
Concentration:
~0.5mg/ml
Immunogen:
Human platelet membranes.
Purity:
Purified
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Specificity:
Recognizes human JAM-1. Activate platelets, leading to aggregation and granule secretion.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.