		Technical Data
J8010-01C4	Junctional Adhesion Molecule 2 (JAM 2, JAM2, JAM-2, C21orf43, CD322, CD322 Antigen, JAM IT/VE JAM, Junction Adhesion Molecule B, JAM B, JAMB, JAM-B, PRO245, Vascular Endothelial Junction Associated Molecule, VE JAM, VEJAM, VE-JAM) (Biotin)

Description:
JAM-2 is a 45kD transmembrane protein a member of the immunoglobulin superfamily otherwise known as CD322. In adult murine tissue CD322 expression is reported to be restricted to high endothelial venules of lymphoid organs, lymphoendothelial cells and endothelial cells in kidney. In humans, CD322 expression is not restricted to high endothelial cells, but is also expressed on human lymphocytes.

Applications:
Suitable for use in Flow Cytometry. Other applications not tested.

Recommended Dilutions:
Flow Cytometry: Neat-1:5; 10ul labels 10e6 cells in 100ul
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Mab	IgG2a	10B1411	Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Mouse	Rat

Concentration:

~0.1mg/ml

Immunogen:

Recombinant soluble JAM-2.

Purity:

Purified by Protein G affinity chromatography.

Form

Supplied as a liquid in PBS, pH 7.4, 1% BSA, 0.09% sodium azide. Labeled with Biotin.

Specificity:

Recognizes mouse junctional adhesion molecule-2 (JAM-2). Species crossreactivity: human

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Aurrand-Lions, M. et al. (2001) JAM-2, a novel immunoglobulin superfamily molecule, expressed by endothelial and lymphatic cells. J. Biol. Chem. 276: 2733 - 2741. 2. Johnson-Léger, C.A. et al. (2002) Junctional adhesion molecule - 2 (JAM-2) promotes lymphocyte transendothelial migration. Blood. 100: 2479 - 2486.