Technical Data
J8010-01C5
Junctional Adhesion Molecule 2 (JAM 2, JAM2, JAM-2, C21orf43, CD322, CD322 Antigen, JAM IT/VE JAM, Junction Adhesion Molecule B, JAM B, JAMB, JAM-B, PRO245, Vascular Endothelial Junction Associated Molecule, VE JAM, VEJAM, VE-JAM) (FITC)
Description:
Junctional adhesion molecule-2 (JAM-2) is a 45kD transmembrane protein a member of the immunoglobulin superfamily otherwise known as CD322. In adult murine tissue CD322 expression is reported to be restricted to high endothelial venules of lymphoid organs, lymphoendothelial cells and endothelial cells in kidney.

Applications:
Suitable for use in Flow Cytometry. Other applications not tested.

Recommended Dilution:
Flow Cytometry: Neat; 10ul labels 10e6 cells in 100ul
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. FITC conjugates are sensitive to light.
TypeIsotypeCloneGrade
MabIgG2a10B1411Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceMouseRat
Concentration:
~0.1mg/ml
Immunogen:
Recombinant soluble JAM-2.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 1% BSA, 0.09% sodium azide. Labeled with fluorescein isothiocyanate (FITC).
Specificity:
Recognizes mouse junctional adhesion molecule-2 (JAM-2). Species crossreactivity: human.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Aurrand-Lions, M. et al. (2001) JAM-2, a novel immunoglobulin superfamily molecule, expressed by endothelial and lymphatic cells. J. Biol. Chem. 276: 2733 - 2741.2. Johnson-Lger, C.A. et al. (2002) Junctional adhesion molecule - 2 (JAM-2) promotes lymphocyte transendothelial migration. Blood. 100: 2479 - 2486.