Technical Data
J8025
Junctional Adhesion Molecule C (JAM-C)
Description:
Junctional adhesion molecule-C (JAM-C) also known as JAM-2 is a 45kD cell adhesion molecule CAM). JAM-C is a transmembrane protein which is a member of the immunoglobulin superfamily found at intercellular junctions of endothelial cells. JAM-C belongs together with JAM-A (JAM or JAM-1) and JAM-B (VE-JAM or JAM-3) to a family of adhesion proteins with a V-C2 immunoglobulin domain organization. JAM plays an important role in tight junctions where it is involved in cell-to-cell adhesion through homophilic interaction. It codistributes with other tight junction components as ZO-1, 7H6 antigen, cingulin and occludin. JAM-C is potentially involved in the junctional sealing of the vascular endothelium, in particular of high endothelial venules (HEV). In adult murine tissue JAM-C expression is reported to be restricted to high endothelial venules of lymphoid organs, lymphoendothelial cells and endothelial cells in kidney. In humans, JAM-C expression is not restricted to endothelial cells, but is also expressed on human lymphocytes.

Applications:
Suitable for use in ELISA, Immunoprecipitation, Flow Cytometry and Immunohistochemistry. Not suitable for Western Blot or Immunohistochemistry on paraffin sections. Other applications have not been tested.

Recommended Dilution:
Flow Cytometry: 1:50
Immunohistochemistry (frozen): 1:50
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG2a6A590Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceMouseRat
Concentration:
~0.1mg/ml
Immunogen:
Recombinant soluble JAM-C
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, 0.1% BSA, 0.02% sodium azide.
Specificity:
Recognizes mouse JAM-C. Species Crossreactivity: Human.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Aurrand-Lions, M., et al., J. Biol. Chem. 276: 2733 (2001). 2. Johnson-Leger, C., et al., Blood 100: 2479 (2002).