Technical Data
J8025
Junctional Adhesion Molecule C (JAM-C)
|
Technical Data |
|
J8025 |
Junctional Adhesion Molecule C (JAM-C) |
|
|
Description: Junctional adhesion molecule-C (JAM-C) also known as JAM-2 is a 45kD cell adhesion molecule CAM). JAM-C is a transmembrane protein which is a member of the immunoglobulin superfamily found at intercellular junctions of endothelial cells. JAM-C belongs together with JAM-A (JAM or JAM-1) and JAM-B (VE-JAM or JAM-3) to a family of adhesion proteins with a V-C2 immunoglobulin domain organization. JAM plays an important role in tight junctions where it is involved in cell-to-cell adhesion through homophilic interaction. It codistributes with other tight junction components as ZO-1, 7H6 antigen, cingulin and occludin. JAM-C is potentially involved in the junctional sealing of the vascular endothelium, in particular of high endothelial venules (HEV). In adult murine tissue JAM-C expression is reported to be restricted to high endothelial venules of lymphoid organs, lymphoendothelial cells and endothelial cells in kidney. In humans, JAM-C expression is not restricted to endothelial cells, but is also expressed on human lymphocytes. Applications: Suitable for use in ELISA, Immunoprecipitation, Flow Cytometry and Immunohistochemistry. Not suitable for Western Blot or Immunohistochemistry on paraffin sections. Other applications have not been tested. Recommended Dilution: Flow Cytometry: 1:50 Immunohistochemistry (frozen): 1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
|
|||||||||||
|
||||||||||||
|
||||||||||||
|
||||||||||||