Technical Data
J9000
Jurkat Cell Lysate
100ul
Biologicals Storage: -20CShipping: Blue Ice
Cellular Protein Preparation:
Cells were lysed in modified RIPA buffer (50mM Tris-HCl, pH 7.4, 1% NP40, 0.25% sodium deoxycholate, 150mM sodium chloride, 1mM EDTA, 1mM PMSF, 1ug/ml aprotinin, 1ug/ml leupeptin, 1ug/ml pepstatin, 1mM sodium orthovanadate, 1mM sodium fluoride). Diluted with non-reducing sample buffer (31mM Tris-HCl, pH 6.8, 5% glycerol, 1% SDS, 0.002% bromophenol blue).

Applications:
Suitable for use in Western Blot as a positive control for antibodies. Other applications not tested

Recommended Usage:
Add 2.5ul 2-mercaptoethanol/100ul of lysate. Boil for 5 minutes to reduce the preparation. Load 20ug of reduced lysate per lane for Western Blot analysis. For use as a positive control for antibodies.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Concentration: ~1mg/ml
Form: Supplied as a liquid in modified RIPA buffer with non-reducing sample buffer.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Dignam, J.D., et al., Nucl. Acids Res. 11 1475-1489 (1983).

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.