Technical Data
K0005-14B
Kallikrein 11 (KLK11, Hippostasin, Trypsin-like serine protease and PRSS20)
Description:
Kallikrein 11 is expressed in brain, skin, ovary and prostate. Increased levels of Kallikrein 11 have been reported in prostate and ovarian carcinoma. It is a serine protease. Kallikrein 11 (KLK11), also known as hippostasin, trypsin-like serine protease and PRSS20, is a member of human tissue kallikrein family.6 Two alternatively spliced forms exist, resulting in 250 (isoform 1) and 282 (isoform 2) amino acid sequences, respectively.1-4. Isoform 1 consists of a signal peptide (residues 1-18), a short pro peptide (residues 19-21) and the mature chain
(residues 22-250). Isoform 2 is identical to isoform 1, except that a 32 amino acid segment is inserted in isoform 2 before residue1 in isoform 1. Isoform 1 is predominantly expressed in brain whereas isoform 2 is preferentially expressed in prostate. KLK11 is a novel marker for ovarian and prostate cancer carcinomas.7-9 rhKLK11, after being activated by thermolysin, is active against a thioester substrate.

Applications:
Suitable for use in ELISA, Western Blot, Immunoprecipitation. Other applications not tested.

Recommended Dilution:
Direct ELISA: 0.5-1.0ug/ml with the appropriate secondary reagents to detect human Kallikrein 11. The detection limit for rhKallikrein 11 is ~1.5ng/well.

Western blot: 0.1-0.2ug/ml with the appropriate secondary reagents to detect human Kallikrein 11. The detection limit for rhKallikrein 11 is approximately 20ng/lane and 5ng/lane under
non-reducing and reducing conditions, respectively.

Immunoprecipitation: Immunoprecipitates rhKallikrein 11 from conditioned media of transfected NS0 cells.

Optimal dilutions to be determined by the researcher.

Recommended Positive Control Tissue:
Ovarian tumor

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceHumanGoat
Concentration:
~0.1mg/ml
Immunogen:
NS0-derived, purified recombinant, human Kallikrein 11, aa 22-250
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in 0.2um filtered solution in PBS with 5% trehalose.
Specificity:
Recognizes human Kallikrein 11 in direct ELISA and Western Blot. 10% crossreactivity with recombinant, human Kallikrein 3 and less than 1% crossreactivity with recombinant, human Kallikrein 5.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Yoshida, S. et al., 1998, Biochim. Biophys. Acta 1399:225 - 228.
2. Yousef, G.M. et al., 2000,Genomics 63:88 - 96.
3. Mitsui, S. et al., 2000, Biochem. Biophys. Res. Commun. 272:205 - 211.
4. Gan, L. et al., 2000, Gene 257:119 - 130.
5. Edwards, K.M. et al., 1999, J. Biol. Chem. 274:30486 - 30473.
6. Yousef, G.M. and E.P. Diamandis, 2001, Endocrine Rev. 22:184 - 204.
7. Diamandis, E.P. et al., 2002, Cancer Res. 62:295 - 300.
8. Nakamura, T. et al., 2003, Urology 61:1042 - 1046.
9. Borgono, C.A. et al., 2003, Int. J. Cancer 106:605 - 610.