Technical Data
K0090-02C
Kappa Light Chain (HCAK1, Ig kappa Chain C Region, IGKC, Immunoglobulin kappa Constant Region, Immunoglobulin kappa Light Chain, Kappa 1 Immunoglobulin Light Chain, Km, MGC111575, MGC62011, MGC72072, MGC88770, MGC88771, MGC88809)
Description:
Useful in the identification of leukemias, plasmacytomas and certain non-Hodgkins lymphomas. The most common feature of these malignancies is the restricted expression of a single light chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant. Immunoglobulin bearing Kappa light chains are present on about 60% of normal B lymphocytes.

Applications:
Suitable for use in ELISA and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Indirect ELISA Titer: 1:50,000
Immunohistochemistry: Frozen or paraffin-embedded sections.
Optimal dilutions to be determined by the researcher.

Hybridoma:
Sp2/0 myeloma cells with spleen cells of Balb/c mice.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG2a5E697Affinity Purified
SizeStorageShippingSourceHost
500ug4C (-20C Glycerol)Blue IceHumanMouse
Concentration:
~2mg/ml
Immunogen:
Mixture of light chains of human immunoglobulins.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide before the addition of glycerol to 40%.
Specificity:
Recognizes the independent constantly expressed epitope of kappa chain. Reacts with free and heavy chain-associated kappa chains.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.