Technical Data
K0090-03
Kappa Light Chain (HCAK1, Ig kappa Chain C Region, IGKC, Immunoglobulin kappa Constant Region, Immunoglobulin kappa Light Chain, Kappa 1 Immunoglobulin Light Chain, Km, MGC111575, MGC62011, MGC72072, MGC88770, MGC88771, MGC88809)
Description:
Useful in the identification of leukemias, plasmacytomas and certain non-Hodgkin’s lymphomas. The most common feature of these malignancies is the restricted expression of a single light chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant. Immunoglobulin bearing Kappa light chains are present on about 60% of normal B lymphocytes.

F(ab')2 Goat anti-rat IgG: RPE (mouse adsorbed)

Fusion Partners: Spleen cells from an immunized PVG.RT1c rat were fused with cells of the rat Y3 Ag.1.2.3 myeloma cell line.

Recommended Negative Controls: Rat IgG1 Negative Control

Applications:
Flow Cytometry: 1:100
Optimal working dilutions to be determined by researcher.

Flow Cytometry: Use 10ul of the suggested working dilution to label 10e6 cells in 100ul.
TypeIsotypeCloneGrade
MabIgG13H2571Purified
SizeStorageShippingSourceHost
1mg-20°CBlue IceMouseRat
Concentration:
~1mg/ml
Immunogen:
Purified mouse IgG.
Purity:
Purified IgG.
Form
Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.
Specificity:
This antibody binds to mouse B lymphocytes, mouse IgM and mouse IgG monoclonal antibodies and recognizes mouse Ig kappa chains. In ELISA assay no binding to mouse lambda chains is seen.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Galfre, G., Milstein, C. & Wright, B. (1979). Nature 277: 131-133. "Rat x rat hybrid myelomas and a monoclonal anti-Fd portion of mouse IgG".