Technical Data
K0090-20
Kappa Light Chain (HCAK1, Ig kappa Chain C Region, IGKC, Immunoglobulin kappa Constant Region, Immunoglobulin kappa Light Chain, Kappa 1 Immunoglobulin Light Chain, Km, MGC111575, MGC62011, MGC72072, MGC88770, MGC88771, MGC88809)
Description:
Useful in the identification of leukemias, plasmacytomas and certain non-Hodgkin’s lymphomas. The most common feature of these malignancies is the restricted expression of a single light chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant. Immunoglobulin bearing Kappa light chains are present on about 60% of normal B lymphocytes.

Applications:
Suitable for use in ELISA and Immunohistochemistry (frozen and paraffin sections, proteolytic treatment with trypsin required).

Recommended Dilution:
Immunohistochemistry: 1:5-1:10; Incubation Time 1 hr at room temperature.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG15F258Supernatant
SizeStorageShippingSourceHost
1ml-20°CBlue IceMouse
Concentration:
Not determined
Immunogen:
Isolated from Bence Jones kappa proteins.
Purity:
Culture supernatant
Form
Supplied as a liquid.
Specificity:
Antigen Recognized Human in Species (tested so far). Recommended for Bone marrow. Reacts with kappa light chains of secreted immunoglobulins and with Ig on the surface of B lymphocytes.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.